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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1268-1274
DOI: https://doi.org/10.20546/ijcmas.2019.810.149


Effect of Light and pH on the Growth of Sclerotium rolfsii in vitro on Collar Rot of Indian Bean
Tushar V. Ghevariya* and P. R. Patel
Department of Plant Pathology, NM College of Agriculture, Navsari Agricultural University, Navsari - 396 450, Gujarat, India
*Corresponding author
Abstract:

Sclerotium rolfsii is one of the most important soil-borne plant pathogen which cause severe loss at the time of seedling development. It also causes collar rot in several crops and wild plants. In this experiment, exposure of pathogen to different light period and pH in order to assess the mycelial growth and number of sclerotia of S. rolfsii was done. For the light experiment three plate continues dark, light and 12 hour interval. All the plates were incubated at 28±1°C. The results reveal that there was no significant difference in mycelial growth and number of sclerotia among them but significant difference was observed when compared with the 12 hour interval. The light condition induces the production of more number of sclerotia than dark condition. In alternative cycles of 12 hour light and 12 hour darkness for ten days resulted in the maximum mycelium growth, more number of sclerotia was also seen when compared with continued light and dark condition. Effect of eight pH levels viz., 5.0, 5.5, 6, 6.5, 7, 7.5, 8 and 8.5 on radial growth and sclerotia formation of S. rolfsii was studied and observations. Mycelium growth was observed at all the pH levels tested but it was maximum at pH 6.5 (87.00mm) after 72 hrs of inoculation. pH 6.0 (85.25mm) and pH 5.5 (83.75mm) were also found favorable. Excellent sclerotia formation was observed at pH 5.5, 6.0 and 6.5 while fair sclerotia production was recorded at pH 5.0, 7.0 and 7.5 and pH 8.0 and 8.5 supported poor sclerotia formation. Highly acidic and alkaline pH is not suitable for the growth of pathogen.


Keywords: Sclerotium rolfsii, pH, Light
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How to cite this article:

Tushar V. Ghevariya and Patel, P. R. 2019. Effect of Light and pH on the Growth of Sclerotium rolfsii in vitro on Collar Rot of Indian Bean.Int.J.Curr.Microbiol.App.Sci. 8(10): 1268-1274. doi: https://doi.org/10.20546/ijcmas.2019.810.149