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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1260-1267
DOI: https://doi.org/10.20546/ijcmas.2019.810.148


Truenat - A Novel Diagnostic Tool for Rapid Detection of Mycobacterium Tuberculosis and Rifampicin Resistance in Pulmonary Samples
J. Vijayalakshmi, A. Surekha, A. Renuka Devi and S. Uma Devi*
Department of Microbiology, Kurnool Medical College, Kurnool, AP. 518002, India
*Corresponding author
Abstract:

Tuberculosis is a major public health problem and second largest cause of death among infectious diseases. Rapid diagnosis of tuberculosis and detection of drug resistance is essential for effective disease control. Control of TB is hindered by the lack of a simple and effective diagnostic tool that can be utilised in resource limited settings. Molecular techniques using Real time PCR technology have gained momentum for rapid diagnosis and detection of drug resistance. To study the prevalence of Pulmonary Tuberculosis and Rifampicin resistance in Kurnool district by using Truenat chip based micro PCR system and to understand the demographical distribution of cases attending the tertiary care hospital. This was a prospective study done during the period from March 2019 to Aug 2019. All the presumptive TB cases with chest symptoms attending Govt.General Hospital, Kurnool were included in the study. Sputum samples were processed by Truenat, a chip based nucleic acid amplification test (Molbio diagnostics pvt. ltd., Goa). The procedure included two steps: first- sputum samples were processed for separation of DNA by using True prep device. Second-Presence of MTB was detected by Truelab™ Real Time micro PCR Analyzer. All the MTB positive samples were tested for the presence of Rifampicin resistance by using another specialized chip. A total of 1825 sputum samples were processed, out of which 368 (20.16%) were positive for MTB. Rifampicin resistance was detected in 70 (19.02%). Positivity was more in males 63.23% as compared to females 36.76%. Majority of the cases were in the age group 40-60 years (46.58%), followed by 20-40 years (33.04%). Truenat is a simple method and laboratory technician with minimal training can perform the procedure. The Turn Around Time (TAT) is approximately one hour. As no special infrastructure or Airconditioning system is required to set up the device it can be used in resource limited settings for rapid diagnosis of MTB and detection of Rifampicin resistance.


Keywords: Chip based assay, Trueprep AUTO Universal Cartridge Based Sample Prep Device TruenatTM MTB chip
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How to cite this article:

Vijayalakshmi, J., A. Surekha, A. Renuka devi and Uma Devi, S. 2019. Truenat - A Novel Diagnostic Tool for Rapid Detection of Mycobacterium Tuberculosis and Rifampicin Resistance in Pulmonary Samples.Int.J.Curr.Microbiol.App.Sci. 8(10): 1260-1267. doi: https://doi.org/10.20546/ijcmas.2019.810.148