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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 977-986
DOI: https://doi.org/10.20546/ijcmas.2019.810.114


Compost Tea Induced Callus Proliferation and Defense Response in Rice (Oryza sativa L.) Callus Cells
B.R. Vanishri* and Veena S. Anil
Department of Plant Biotechnology, UAS, GKVK, Bangalore 560065, Karnataka, India
*Corresponding author
Abstract:

Callus growth and vitality is one of the important prerequisites for further success of in vitro studies. In vitro calli systems can also act as a model system in understanding defense responses at the cellular level. This study investigated the efficiency of Compost tea (CT) in inducing defense response and as an organic supplement in vitro callus growth in rice. Compost tea is an organic liquid product derived from quality compost, carrying useful microorganism and molecules capable of protecting and stimulating the growth of the plants. Morphological and Biochemical responses of rice calli inoculated onto Murashige and skoog medium supplemented with CT in the presence or absence of 2,4-D showed significantly high callus proliferation, fresh weight of calli, Peroxidase (POX) and Superoxide dismutase (SOD) activity after 15 days of inoculation as compared to controls. Interestingly MS media supplemented only on MS media supplemented with CT and without 2, 4 D showed rooting/root like growth from calli. The observed callus proliferation, and root like growth suggesting presence of auxin like molecules in compost tea. The study shows that compost tea can induce a cellular defense response. Secondly under the light of the findings, compost tea can be used as an organic bio stimulant for efficient callus growth and complementing commercial chemical hormones in rice.


Keywords: Compost tea, Callus, Peroxidase, Superoxide dismutase
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How to cite this article:

Vanishri, B.R. and Veena S. Anil. 2019. Compost Tea Induced Callus Proliferation and Defense Response in Rice (Oryza sativa L.) Callus Cells.Int.J.Curr.Microbiol.App.Sci. 8(10): 977-986. doi: https://doi.org/10.20546/ijcmas.2019.810.114