National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Callus growth and vitality is one of the important prerequisites for further success of in vitro studies. In vitro calli systems can also act as a model system in understanding defense responses at the cellular level. This study investigated the efficiency of Compost tea (CT) in inducing defense response and as an organic supplement in vitro callus growth in rice. Compost tea is an organic liquid product derived from quality compost, carrying useful microorganism and molecules capable of protecting and stimulating the growth of the plants. Morphological and Biochemical responses of rice calli inoculated onto Murashige and skoog medium supplemented with CT in the presence or absence of 2,4-D showed significantly high callus proliferation, fresh weight of calli, Peroxidase (POX) and Superoxide dismutase (SOD) activity after 15 days of inoculation as compared to controls. Interestingly MS media supplemented only on MS media supplemented with CT and without 2, 4 D showed rooting/root like growth from calli. The observed callus proliferation, and root like growth suggesting presence of auxin like molecules in compost tea. The study shows that compost tea can induce a cellular defense response. Secondly under the light of the findings, compost tea can be used as an organic bio stimulant for efficient callus growth and complementing commercial chemical hormones in rice.
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