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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 916-927
DOI: https://doi.org/10.20546/ijcmas.2019.810.107


Molecular Characterization of Major Types of Staphylococcal Cassette Chromosome mec (SCCmec) in Methicillin-Resistant Staphylococci (MRS) from Animal and Human Origin
S.N. Ghodasara1*, J.H. Purohit2, A.R. Baria3, A.S. Patel3, B.B. Javia1, D.B. Barad1, J.A. Kathiria4 and A.R. Bhadania5
1Department of Veterinary Microbiology, College of Veterinary Science and A. H., Junagadh Agricultural University, Junagadh, Gujarat, India
2Department of Veterinary Microbiology, College of Veterinary Science and A. H., Anand Agricultural University, Anand, Gujarat, India
3Department of Livestock Products Technology, College of Veterinary Science and A. H., Junagadh Agricultural University, Junagadh, Gujarat, India
4Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and A. H., Junagadh Agricultural University, Junagadh, Gujarat, India
5Department of Veterinary Pathology, College of Veterinary Science and A. H., Junagadh Agricultural University, Junagadh, Gujarat, India
*Corresponding author
Abstract:

A total of 202 animal samples (167 milk and 35 pus/exudate samples from cattle and buffalo) were collected and examined from dairy cattle and buffaloes. Simultaneously, 100 nasal swabs were collected from the closely associated personnel and farm workers. Out of these, the Staphylococcus spp. were isolated from86 (42.57%) animal and 62 (62%) human samples. Of these, total 9 isolates from animals and 20 isolates from human were identified as MRS positive. Out of 9 and 20 MRS isolates from animal and human, 1 (11.11%) and 2 (10%) isolate were Methicillin-Resistant Coagulase Positive Staphylococcus (MRCoPS), whereas 8 (88.89%) and 18 (90%) isolates were Methicillin-Resistant Coagulase Negative Staphylococcus (MRCoNS), respectively. The SCCmec typing of 16 and 40 MRS (Including departmental isolates) isolates from animal and human were carried out. Out of these, the study revealed 18.75% (3/16) and 25% (10/40) isolates were classified as hospital associated methicillin-resistant staphylococci (HA-MRS), whereas 68.75% (11/16) and 45% (18/40) isolates were classified as community associated methicillin resistant staphylococci (CA-MRS) and 12.5% (2/16) and 30% (12/40) MRS isolates remained untypable, respectively. The overall percentage of CA-MRS (63.04%) was higher as compare to HA-MRS (28.26%) among both the species.


Keywords: SCCmec, Methicillin, Community associated, Hospital associated, Nasal swab
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How to cite this article:

Ghodasara, S.N., J.H. Purohit, A.R. Baria, A.S. Patel, B.B. Javia, D.B. Barad, J.A. Kathiria and Bhadania, A.R. 2019. Molecular Characterization of Major Types of Staphylococcal Cassette Chromosomemec (SCCmec) in Methicillin-Resistant Staphylococci (MRS) from Animal and Human Origin.Int.J.Curr.Microbiol.App.Sci. 8(10): 916-927. doi: https://doi.org/10.20546/ijcmas.2019.810.107