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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 809-816
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.090


Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection
Ghada E. Amr1*, Dina M. Atef1 and Rabab Elbehedy2
1Departments of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt
2Pediatrics, Faculty of Medicine, Zagazig University, Egypt
*Corresponding author
Abstract:

Human bocavirus (HBoV) was first identified as parvovirus in 2005 in nasopharyngeal aspirates of children with lower respiratory tract infection. The objectives of this study were to determine HBoV as a cause of acute lower respiratory tract infection(ALRTI) in children by investigating the presence of HBoV DNA, to evaluate diagnostic efficiency of HBov IgM assay in the serum of the studied patients and to assess the impact of the viral load on the clinical manifestations of children with positive HBoV DNA samples. We investigated 123-nasopharyngeal aspirate samples of hospitalized children with ALRTI by Real-time PCR for detection of HBoV DNA and estimating its viral load. Estimation of immunoglobulin M (IgM) by ELISA in patient’s serum samples was another method used for diagnosis of HBoV infection. There were 19 patients with positive nasopharyngeal aspirate samples by PCR among them there were 16 serum samples positive for HBoV IgM by ELISA (sensitivity was 84.2%). Longer hospital stay and severe ALRTI signs were detected in children samples with high viral load (16 samples).


Keywords: Human bocavirus, Lower respiratory tract infection in children, Real-time PCR, Serology.
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How to cite this article:

Ghada E. Amr, Dina M. Atef and Rabab Elbehedy. 2017. Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection.Int.J.Curr.Microbiol.App.Sci. 6(2): 809-816. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.090