National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The protocol for high frequency induction of callus from nucellus tissue was re initiation attained in polyembryonic mango cultivars Vellaikolumban and Olour. Among different sterilization treatments followed, dipping fruits in 70 % ethanol +followed by treating them in Cetrimide 1000 ppm - 15 minutes further treating with NaOCl - 1 % +Tween 20 (one drop per 100 ml) for 15 minutes and Flame sterilisation under laminar air flow found to be the best treatment. Among the basal media, Rugini Olive (RO) medium followed by Murashige and Skoog (MS) medium was superior to Gamborg's B5 (B5) for culture establishment. Among various physiological stages of fruits used for culturing, 30-40 days old and 40-50 days old fruits found to ideal for nucellar embryogenesis in cv. Vellaikolumban and cv. Olour respectively. Embryogenic calli formed on establishment medium (RO, 6 percent sucrose, 1g activated charcoal, 2.5 g/L phytagel, 5 mg each of 2,4-D and GA3).This calli can be used for further steps in somatic embryogenesis.
Citations |
 |
 |
 |
 |
