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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 686-692
DOI: https://doi.org/10.20546/ijcmas.2019.810.078


Factors Affecting Induction of Callus from Nucellus Tissue of Polyembryonic Mango Cv. Vellaikolumban and Cv. Olour
S. Sajana1*, P. Thomas2, P. Nandeesha2 and Reju M Kurian3*
1ICAR- IARI, Outreach Campus, IIHR, Bangalore, India
2Division of Biotechnology, 3Division of Fruit crops, ICAR-IIHR, Bengaluru, India
*Corresponding author
Abstract:

The protocol for high frequency induction of callus from nucellus tissue was re initiation attained in polyembryonic mango cultivars Vellaikolumban and Olour. Among different sterilization treatments followed, dipping fruits in 70 % ethanol +followed by treating them in Cetrimide 1000 ppm - 15 minutes further treating with NaOCl - 1 % +Tween 20 (one drop per 100 ml) for 15 minutes and Flame sterilisation under laminar air flow found to be the best treatment. Among the basal media, Rugini Olive (RO) medium followed by Murashige and Skoog (MS) medium was superior to Gamborg's B5 (B5) for culture establishment. Among various physiological stages of fruits used for culturing, 30-40 days old and 40-50 days old fruits found to ideal for nucellar embryogenesis in cv. Vellaikolumban and cv. Olour respectively. Embryogenic calli formed on establishment medium (RO, 6 percent sucrose, 1g activated charcoal, 2.5 g/L phytagel, 5 mg each of 2,4-D and GA3).This calli can be used for further steps in somatic embryogenesis.


Keywords: Heterozygosity, Micro propagation, Somatic embryogenesis, Nucellus tissue
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How to cite this article:

Sajana, S., P. Thomas, P. Nandeesha and Reju M Kurian. 2019. Factors Affecting Induction of Callus from Nucellus Tissue of Polyembryonic Mango Cv. Vellaikolumban and Cv. Olour.Int.J.Curr.Microbiol.App.Sci. 8(10): 686-692. doi: https://doi.org/10.20546/ijcmas.2019.810.078