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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 436-441
DOI: https://doi.org/10.20546/ijcmas.2019.810.046


Genetic Diversity Profiles of Salmonella typhimurium Isolated from Foods of Animal Origin
Yasarla suresh, Chinnam Bindhu kiranmayi, Thummati Srinivasa Rao, M. Srivani and Metta muralidhar
Department of Veterinary Publichealth and epidemiology, N.T.R. C.V.Sc, Gannavaram, India
*Corresponding author
Abstract:

Salmonella is a foodborne pathogen having a worldwide public health concern. The present study was undertaken to characterize Salmonella typhimurium of animal origin based on cultural isolation and genetic diversity studies by employing ERIC-PCR and REP-PCR. A total of 516 samples comprising poultry cloacal swabs (249), raw foods of animal origin (118 chicken samples, 65 mutton and 30 pork), 17 poultry liver swabs and 37 poultry farm water samples were examined for presence of Salmonella serovars. A total of 21 Salmonella were isolated among them, 7 were S. typhimurium. For all 7 isolates REP and ERIC PCR were employed to study genetic diversity among Salmonella isolates. ERIC PCR and REP-PCR analysis revealed a greater degree of heterogeneity among S. typhimurium isolates from different sources. ERIC-PCR and REP-PCR genotyping distinguished 7 isolates. The discriminatory power of ERIC-PCR and REP-PCR for Salmonella typhimurium isolates was found to be highly significant (>0.9) i.e. 1.0, for both.


Keywords: Enterobacteriaceae, Gram-negative, Lactose non fermenting, Rod-shaped bacteria
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How to cite this article:

Yasarla Suresh, Chinnam Bindhu kiranmayi, Thummati Srinivasa Rao, M. Srivani and Metta Muralidhar. 2019. Genetic Diversity Profiles of Salmonella typhimurium Isolated from Foods of Animal Origin.Int.J.Curr.Microbiol.App.Sci. 8(10): 436-441. doi: https://doi.org/10.20546/ijcmas.2019.810.046