National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Salmonella is a foodborne pathogen having a worldwide public health concern. The present study was undertaken to characterize Salmonella typhimurium of animal origin based on cultural isolation and genetic diversity studies by employing ERIC-PCR and REP-PCR. A total of 516 samples comprising poultry cloacal swabs (249), raw foods of animal origin (118 chicken samples, 65 mutton and 30 pork), 17 poultry liver swabs and 37 poultry farm water samples were examined for presence of Salmonella serovars. A total of 21 Salmonella were isolated among them, 7 were S. typhimurium. For all 7 isolates REP and ERIC PCR were employed to study genetic diversity among Salmonella isolates. ERIC PCR and REP-PCR analysis revealed a greater degree of heterogeneity among S. typhimurium isolates from different sources. ERIC-PCR and REP-PCR genotyping distinguished 7 isolates. The discriminatory power of ERIC-PCR and REP-PCR for Salmonella typhimurium isolates was found to be highly significant (>0.9) i.e. 1.0, for both.
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