National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Gut microbiota has been proposed as a new environmental risk factor responsible for obesity. So, the aim of this study was to assess the gut microbiota profile, with special consideration to Lactobacilli, E. coli and S. aureus viable bacterial count (VBC) in (CFU/gm) and their association with body mass index (BMI) and obesity using quantitative stool culture. This study was conducted on 100 individuals; 74 with disturbed BMI who were divided into subgroups, with no other associated co-morbidities and 26 healthy average weight volunteers. Fresh stool samples were collected and cultured directly on MacConkey, xylose lysine deoxycholate, Blood and Man-Rogoza Sharpe agar and quantitatively with serial dilutions (10-2 – 10-9) in sterile normal saline. S. aureus was not isolated in any group, while, Lactobacilli and E. coli were isolated from all groups. Lactobacilli have a significant low VBC in obese and the highest VBC in the normal weight group showing a negative correlation with BMI. While, E. coli had a significantly high VBC in obese and the lowest in the underweight group showing a positive correlation with BMI. In conclusion, low Lactobacilli VBC and high E. coli VBC are significantly related to increased BMI and thus obesity.
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