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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 393-400
DOI: https://doi.org/10.20546/ijcmas.2019.810.041


Molecular Characterization of Soybean Genotypes in Response to Charcoal Rot Disease by using SSR Markers
S. V. Chavan1*, P. V. Jadhav2, M. S. Madke2, S. S. Mane3 and R. S. Nandanwar1
1Department of Agricultural Botany, Dr. PDKV, Akola, India
2Department of Agricultural Biotechnology, Dr. PDKV, Akola, India
3Department of Plant Pathology, Dr. PDKV, Akola, India
*Corresponding author
Abstract:

Charcoal rot (CR) disease caused by Macrophomina phaseolina is responsible for signi´Čücant yield losses in soybean production. Among the methods available for controlling this disease, breeding for resistance is the most promising. The present study helped to evaluate soybean genotypes for identifying promising genotypes which proved to be resistant to charcoal rot. The present study was carried out at Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during the year 2018-19 to evaluate various genotypes of soybean for charcoal rot resistance. Charcoal rot disease caused by Macrophomina phaseolina is one of the most damaging diseases of soybean resulting to 70 % losses and till date no immune genotype is known for the same. Molecular characterization of these genotypes was done by using SSR markers. Molecular profiles revealed remarkable polymorphism and observations showed that in total 143 amplicons were tested with an average of 6.22 alleles per locus. Out of the total screened alleles 49 were monomorphic alleles with an average of 2.13 and 94 were polymorphic alleles with an average of 4.09. Results showed an average of 65.97 polymorphism percent. The PIC (Polymorphic information content) value of 23 microsatellite loci ranged from 0.30 to 0.84 with an average value of 0.70,these studies will help in mapping studies and breeding program for development of charcoal rot resistance in soybean genotypes which will be of utmost importance.


Keywords: Soybean, Charcoal rot, Inheritance, SSR, Validation
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How to cite this article:

Chavan, S. V., P. V. Jadhav, M. S. Madke, S. S. Mane and Nandanwar, R. S. 2019. Molecular Characterization of Soybean Genotypes in Response to Charcoal Rot Disease by using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 8(10): 393-400. doi: https://doi.org/10.20546/ijcmas.2019.810.041