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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 361-373
DOI: https://doi.org/10.20546/ijcmas.2019.810.037


Standardisation of Gamma Dose in Papaya cv. Ranchi Local and Arka Surya
Prativa Sahu1*, D. K. Dash2, J. Lenka3, S. N. Dash2, S. K. Tripathy2, A. Mishra2 and A. Sahu4
1Department of Fruit Science, ICAR- Indian Institute of Water Management,
C.S.Pur, Bhubaneswar, Odisha-751023, India
2College of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha-751003, India
3ICAR- Central Institute for Subtropical Horticulture, Rehmankhera, P.O. Kakori, Lucknow, Uttar Pradesh 226101, India
4ICAR- Central Institute for Women in Agriculture, Bhubaneswar, Odisha – 751003, India
*Corresponding author
Abstract:

Gamma radiation is the most commonly used mutagen in plants as it causes a high frequency of induced mutations. It can be used to overcome the difficulties in papaya breeding to get true to type plantlets especially in dioecious varieties. An optimum dose is highly desired to produce the high frequency of mutations with minimum killing of treated individuals. Therefore, the present investigation was undertaken to determine the LD50 of gamma rays and effect of different dosages of gamma rays on morphological, physio-chemical, biochemical parameters of papaya. In the preliminary dosimetry study, seeds from two papaya varieties, Ranchi Local and Arka Surya were irradiated in a gamma chamber at Bhabha Atomic Research Centre (BARC), Mumbai with 7 different radiation doses ranging from 10 Gy to 70 Gy at every interval of 10 Gy. Prior to irradiation in one experiment, seeds were soaked overnight in water and surface-dried and in another experiment, seeds were immersed in water while irradiation. Radio sensitivity test of germinated seeds showed that the LD50 value was 28.35 and 33.13 Gy for pre-soaked seeds & 24.05 and 23.78 Gy for seeds immersed in water for Ranchi Local & Arka Surya variety respectively. The GR50 was thus calculated to be 31.64 and 37.50 Gy for pre-soaked seeds & 28.28 and 30.11 Gy for seeds immersed in water for Ranchi Local & Arka Surya variety respectively. From the analysis of GR50 for both the irradiated conditions, it was found that 30 Gy (for pre-soaked seeds) and 20 Gy (for seeds immersed in water) was the most effective dose with maximum possibility of desirable mutation.


Keywords: Papaya, Gamma rays, LD50, germination, GR50, GR30
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How to cite this article:

Prativa Sahu, D. K. Dash, J. Lenka, S. N. Dash, S. K. Tripathy, A. Mishra and Sahu, A. 2019. Standardisation of Gamma Dose in Papaya cv. Ranchi Local and Arka Surya.Int.J.Curr.Microbiol.App.Sci. 8(10): 361-373. doi: https://doi.org/10.20546/ijcmas.2019.810.037