National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present investigation an attempt was carry out to test the pathogenicity of the sixteen isolates of C. capsici obtained from different districts of Madhya Pradesh on ripe and green chilli by pin prick method and the genetically variation of these isolates was also detected by Random Amplified Polymorphic DNA (RAPD) technique. On ripe chilli, significantly maximum anthracnose incidence was recorded in BHP-1 (56.60 %) and minimum anthracnose incidence was recorded in CHN-2 (33.80 %) whereas on green chilli, significantly maximum anthracnose incidence was recorded in JHB-2 (89.60 %) while minimum anthracnose incidence was recorded in CHN-2 (61.40 %). For genetic variability, two primers were used which produced 32 scorable bands in which 23 bands were polymorphic and showed 72 % polymorphism. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into two clusters (I and II), confirming the genetic diversity among the isolates of C. capsici. The genetical similarity coefficient value ranged from 0.335 – 0.992 for OPD 07 primer across 7 isolates of C. capsici. RAPD analysis showed a clear difference in different isolates of C. capsici.
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