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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 303-312
DOI: https://doi.org/10.20546/ijcmas.2019.810.031


RAPD Analysis of Colletotrichum capsici Causing Anthracnose of Chilli in Madhya Pradesh, India
Ashish Bobade1*, P. P. Shastry2, Ajay Kumar3, R. K. Pandya3, Sushma Tiwari4 and Reeti Singh3
1Krishi Vigyan Kendra, B.M. College of Agriculture, Khandwa-450001(MP), India
2Zonal Agricultural Research Station, Khargone-451001(MP), India
3Department of Plant Pathology, College of Agriculture, Gwalior-474002 (M.P.), India
4Department of Plant Breeding and Genetics, College of Agriculture,
Gwalior-474002 (M.P.), India
*Corresponding author
Abstract:

In the present investigation an attempt was carry out to test the pathogenicity of the sixteen isolates of C. capsici obtained from different districts of Madhya Pradesh on ripe and green chilli by pin prick method and the genetically variation of these isolates was also detected by Random Amplified Polymorphic DNA (RAPD) technique. On ripe chilli, significantly maximum anthracnose incidence was recorded in BHP-1 (56.60 %) and minimum anthracnose incidence was recorded in CHN-2 (33.80 %) whereas on green chilli, significantly maximum anthracnose incidence was recorded in JHB-2 (89.60 %) while minimum anthracnose incidence was recorded in CHN-2 (61.40 %). For genetic variability, two primers were used which produced 32 scorable bands in which 23 bands were polymorphic and showed 72 % polymorphism. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into two clusters (I and II), confirming the genetic diversity among the isolates of C. capsici. The genetical similarity coefficient value ranged from 0.335 – 0.992 for OPD 07 primer across 7 isolates of C. capsici. RAPD analysis showed a clear difference in different isolates of C. capsici.


Keywords: Chilli, Colletotrichum capsici, Pathogenicity, RAPD, Molecular variability
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How to cite this article:

Ashish Bobade, P. P. Shastry, Ajay Kumar, R. K. Pandya, Sushma Tiwari and Reeti Singh 2019. RAPD Analysis of Colletotrichum capsici Causing Anthracnose of Chilli in Madhya Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 8(10): 303-312. doi: https://doi.org/10.20546/ijcmas.2019.810.031