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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 169-178
DOI: https://doi.org/10.20546/ijcmas.2019.810.017


Effect of Zinc Oxide Nanoparticles (ZnO NP) on Antioxidant Status of Methotrexate (MTX) Induced Toxicity in Wistar Albino Rats
Akshata S. Angadi1*, Suguna Rao1, M. L. Satyanarayana1,H. D. Narayanaswamy1, S. M. Byregowda2 and G. B. Manjunatha Reddy3
1Department of Veterinary Pathology, VeterinaryCollege, Bangalore, KVAFSU, Bidar, India
2Institute of Animal Health and Veterinary Biologicals, Bangalore, KVAFSU, Bidar, India
3National Institute of Veterinary Epidemiology and Disease Informatics, Bangalore, India
*Corresponding author
Abstract:

The experiment was undertaken to evaluate the effect of zincoxide nanoparticles (ZnO NPs) on antioxidant status of methotrexate intoxicated Wistar albino rats. Seventy-two rats of 180 to 200g weight were equally divided into six groups. MTX was used at the rate of 5mg/kg b.w intraperitoneally for three consecutive days to induce toxicity and ZnO NPs was used @ of 50mg/kg b.w through oral gavage for 45 days. Antioxidant status of rats was evaluated by estimating products of oxidative injury (MDA) and endogenous antioxidant enzymes (CAT, SOD and GPx) in the liver on 7th, 21st and 45th days of experiment. All the antioxidant enzymes markedly reduced in MTX positive control with increased levels of MDA. In ZnO NPs positive control, the enzymes were numerically higher than normal control with mild increase in MDA levels at later stages of experiment. The MTX and ZnO NPs treated rats showed improved antioxidant status and decreased MDA levels than MTX alone intoxicated rats. ZnONPs pre-treated rats showed improved antioxidant profile than ZnO NPs concurrent treatment and were comparable with that of a proven herbal hepato-protectant ‘Silymarin’. In conclusion, the experiment suggested that implementation of ZnO NP scan combat MTX toxicity.


Keywords: Methotrexate, Zinc oxide nanoparticles, Endogenous antioxidant enzymes, Malondialdehyde, Oxidative injury
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How to cite this article:

Akshata S. Angadi, Suguna Rao, M. L. Satyanarayana, H. D. Narayanaswamy, S. M. Byregowda and Manjunatha Reddy, G. B. 2019. Effect of Zinc Oxide Nanoparticles (ZnO NP) on Antioxidant Status of Methotrexate (MTX) Induced Toxicity in Wistar Albino Rats.Int.J.Curr.Microbiol.App.Sci. 8(10): 169-178. doi: https://doi.org/10.20546/ijcmas.2019.810.017