National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Corynebacterium sp. is a Gram-positive, non-motile and facultatively anaerobic bacilli present in the environment as ubiquitous organism. A 45 day-old Holstein Friesian calf from University Livestock Farm (ULF), Kerala Veterinary and Animal Sciences University, Mannuthy was reported with joint ill in both of its forelimbs. Using aseptic procedures, synovial fluid was collected from the right knee joint and inoculated on to blood agar and incubated at 37â°C for 24 to 48 hrs to isolate and identify the bacterial aetiology involved. Pin-point gray colonies with hazy haemolysis could be observed. On Gram’s staining, Gram-positive bacilli with palisade arrangement could be observed. On sub culturing on to Potassium tellurite blood agar, black coloured colonies suggestive of Corynebacterium sp. was observed. Biochemical tests were performed both by conventional method and using Vitek system and the organism was identified as Corynebacterium jeikeium. On antibiotic sensitivity test (ABST), the organism was found to be highly sensitive to gentamicin, and highly resistant to ampicillin. For molecular confirmation, rpo B gene specific polymerase chain reaction (PCR) was performed and an expected 452 bp amplicons was obtained. Hence, the present case reports the isolation and characterisation of Corynebacterium jeikeium from a case of joint ill in a calf using conventional and molecular methods.
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