Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2875-2880
DOI: https://doi.org/10.20546/ijcmas.2019.809.330


Isolation and Identification of Corynebacterium jeikeium from Synovial Fluid of a Joint Ill Affected Calf
Nair Aswathy1, P. M. Priya1*, R. Ambily1, E. Niyas2, Sudheesh S. Nair3, Rinsha Balan1 and M. Mini1
1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India
2ULFFRDS, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India
3Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India
*Corresponding author
Abstract:

Corynebacterium sp. is a Gram-positive, non-motile and facultatively anaerobic bacilli present in the environment as ubiquitous organism. A 45 day-old Holstein Friesian calf from University Livestock Farm (ULF), Kerala Veterinary and Animal Sciences University, Mannuthy was reported with joint ill in both of its forelimbs. Using aseptic procedures, synovial fluid was collected from the right knee joint and inoculated on to blood agar and incubated at 37⁰C for 24 to 48 hrs to isolate and identify the bacterial aetiology involved. Pin-point gray colonies with hazy haemolysis could be observed. On Gram’s staining, Gram-positive bacilli with palisade arrangement could be observed. On sub culturing on to Potassium tellurite blood agar, black coloured colonies suggestive of Corynebacterium sp. was observed. Biochemical tests were performed both by conventional method and using Vitek system and the organism was identified as Corynebacterium jeikeium. On antibiotic sensitivity test (ABST), the organism was found to be highly sensitive to gentamicin, and highly resistant to ampicillin. For molecular confirmation, rpo B gene specific polymerase chain reaction (PCR) was performed and an expected 452 bp amplicons was obtained. Hence, the present case reports the isolation and characterisation of Corynebacterium jeikeium from a case of joint ill in a calf using conventional and molecular methods.


Keywords: Calf, Corynebacterium jeikeium, joint ill, polymerase chain reaction, rpo B gene
Download this article as Download

How to cite this article:

Nair Aswathy, P. M. Priya, R. Ambily, E. Niyas, Sudheesh S. Nair, Rinsha Balan and Mini M. 2019. Isolation and Identification of Corynebacterium jeikeium from Synovial Fluid of a Joint Ill Affected Calf.Int.J.Curr.Microbiol.App.Sci. 8(9): 2875-2880. doi: https://doi.org/10.20546/ijcmas.2019.809.330