Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2869-2874
DOI: https://doi.org/10.20546/ijcmas.2019.809.329


Isolation and Characterization of Indigenous Probiotic Bacteria from Silkworm (Bombyx mori L.) Gut
M. Saranya1*, S. V. Krishnamoorthy1, D. Balachandar2 and M. Tilak3
1Department of Sericulture, Forest College and Research Institute, Mettupalayam, India
2Department of Agrl. Microbiology, TNAU, Coimbatore, India
3Department of Forest products and utilization, Forest College and Research Institute, Mettupalayam, India
*Corresponding author
Abstract:

Lactic acid bacteria are described as heterogeneous group of regular, gram-positive, rod shaped, non-motile, non-spore forming bacteria with absence of catalase enzyme. Laboratory study was undertaken to isolate and characterize the lactic acid bacteria from silkworm gut in phenotype basis. A total of 21 silkworm gut samples were collected from silkworm breeds, Double hybrid {(CSR 6 X CSR 26) X (CSR 2 X CSR 27)} obtained from Chawki rearing center (CRC) at Annur, Coimbatore. The bacteria samples were isolated after growth on MRS agar medium and pure culture was obtained by sub-culturing on the same medium. Purity of each culture was confirmed by morphological investigation, Gram’s staining and further identification were done by lactic acid bacteria through specific biochemical tests. Finally eight bacterial isolates (SWGL 6, SWGL 7, SWGL 8, SWGL 9, SWGL 10, SWGL 11, SWGL 23 and SWGL 30) were identified as lactic acid bacteria group after morphological, cultural and biochemical characterization.


Keywords: Silkworm gut samples, culture and biochemical characteristics and lactic acid bacteria
Download this article as Download

How to cite this article:

Saranya M., S. V. Krishnamoorthy, D. Balachandar and Tilak M. 2019. Isolation and Characterization of Indigenous Probiotic Bacteria from Silkworm (Bombyx mori L.) Gut.Int.J.Curr.Microbiol.App.Sci. 8(9): 2869-2874. doi: https://doi.org/10.20546/ijcmas.2019.809.329