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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2783-2787
DOI: https://doi.org/10.20546/ijcmas.2019.809.320


Isolation and Identification of Bacillus cereus from Milk and Milk Products in Udaipur, Rajasthan, India
Subhash Chand Meena1*, Abhishek Gaurav1, S.S. Shekhawat1, Bincy Joseph2, Hitesh Kumar1 and Nirmal Kumar1
1Department of Veterinary Public Health,
2Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan, India
*Corresponding author
Abstract:

The present study was envisaged with the aim to isolate and identify Bacillus cereus from milk and milk products. A total of 160 samples which comprise of raw pooled market milk (n=20), pasteurized milk (n=20), dahi (n=20), paneer (n=20), khoa (n=20), milk powder (n=20), ice cream (n=20) and butter (n=20) were collected and processed in the laboratory. Out of the 160 samples screened, Bacillus cereus could be isolated from 44 samples of different milk and milk products employing culture and biochemical assays. Also, the representative phenotypically confirmed isolates (n=10) were further subjected for genotypic confirmation by using PCR. On molecular analysis, gyrB gene could be detected in 100% (10/10) isolates, while 60% (6/10) and 40% (4/10) of the isolates were found positive for the cytK and hblA genes, respectively. Presence of enterotoxigenic genes (cytK and hblA) in the isolates possesses a potential health threat for the public. Keeping in the view, there is an insistent need for elaborative study with more number of samples from different part of the region.


Keywords: Bacillus cereus, Biochemical assay, gyrB gene, cytK gene, hblA gene
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How to cite this article:

Subhash Chand Meena, Abhishek Gaurav, S.S. Shekhawat, Bincy Joseph, Hitesh Kumar and Nirmal Kumar. 2019. Isolation and Identification of Bacillus cereus from Milk and Milk Products in Udaipur, Rajasthan, India.Int.J.Curr.Microbiol.App.Sci. 8(9): 2783-2787. doi: https://doi.org/10.20546/ijcmas.2019.809.320