National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was envisaged with the aim to isolate and identify Bacillus cereus from milk and milk products. A total of 160 samples which comprise of raw pooled market milk (n=20), pasteurized milk (n=20), dahi (n=20), paneer (n=20), khoa (n=20), milk powder (n=20), ice cream (n=20) and butter (n=20) were collected and processed in the laboratory. Out of the 160 samples screened, Bacillus cereus could be isolated from 44 samples of different milk and milk products employing culture and biochemical assays. Also, the representative phenotypically confirmed isolates (n=10) were further subjected for genotypic confirmation by using PCR. On molecular analysis, gyrB gene could be detected in 100% (10/10) isolates, while 60% (6/10) and 40% (4/10) of the isolates were found positive for the cytK and hblA genes, respectively. Presence of enterotoxigenic genes (cytK and hblA) in the isolates possesses a potential health threat for the public. Keeping in the view, there is an insistent need for elaborative study with more number of samples from different part of the region.
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