Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2565-2578
DOI: https://doi.org/10.20546/ijcmas.2019.809.297


Scalable Production of Collagen Isolated from Streptococcus pyogenes
R. M. Akshaya and Priya R. Iyer*
Department of Biotechnology Women`s Christian College Chennai-600006, India
*Corresponding author
Abstract:

Streptococcus pyogenes was isolated from the throat swabs of infected individual and swabbed on the blood agar and sodium azide crystal violet blood agar plates. Isolated organism was grown on the semi-synthetic medium for enrichment and extraction of M protein. The extracted M protein was further purified. The purified M protein was estimated by techniques like Lowry’s method of protein estimation. The M protein was subjected to confirmation techniques and SDS-PAGE analysis. The optimum conditions for the production of M protein like pH, temperature, glucose concentrations were standardized by analyzing the growth of M protein at different pH, temperature and glucose concentrations. Fermentation of collagen containing source with Streptococcus pyogenes and Streptococcal M protein in a culture medium was carried out at different conditions. The amount of collagen production before fermentation and after fermentation was estimated and confirmed. Amount of collagen production increased was estimated and confirmed by thin layer chromatography and SDS-PAGE analysis.


Keywords: Collagen, Streptococus pyogene
Download this article as Download

How to cite this article:

Akshaya R. M. and Priya R. Iyer 2019. Scalable Production of Collagen Isolated from Streptococcus pyogenes.Int.J.Curr.Microbiol.App.Sci. 8(9): 2565-2578. doi: https://doi.org/10.20546/ijcmas.2019.809.297