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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2540-2545
DOI: https://doi.org/10.20546/ijcmas.2019.809.294


ISSR-Based Molecular Characterization of Opium poppy (Papaver somniferum L.) Genotypes
Prateek Sharma1*, Arunabh Joshi1, Ganesh Rajamani1, Devendra Jain1, R. S. Choudahary2, Amit Dadheech3, Deepak Sharma1, Suresh Rajoriya1 and Garima Sharma1
1Department of Molecular Biology and Biotechnology,
2Department of Agronomy,
3Department of Genetics and Plant Breeding, Rajasthan College of agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur-313001, India
*Corresponding author
Abstract:

Opium poppy (Papaver somniferum L.) is an important medicinal crop in the world. In the present investigation 24 prominent opium accessions were assessed by ISSR molecular markers for the genetic diversity analysis. A total of 116 amplified bands were obtained from the 15 primers, out of which 98 were polymorphic. Overall size of PCR amplified products had ranged between 300 bp to 2800 bp. The polymorphism percentage ranged from 16.66% to 100% for nine primers. The ISSR similarity matrix revealed the Jaccard’s similarity values lie between 0.35-0.90. The 24 genotypes could be divided into two major clusters at a similarity coefficient of 0.47, Chetak Aphim sole genotype made single cluster while Cluster II included 23 genotypes. UPGMA method used for dendrogram exhibited the proper genetic diversity in all the 24 accessions of opium poppy. The present investigation revealed that significant diversity existed in all the genotypes of Opium poppy.


Keywords: Molecular marker, ISSR, Papaver somniferum L. Diversity
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How to cite this article:

Prateek Sharma, Arunabh Joshi, Ganesh Rajamani, Devendra Jain, R. S. Choudahary, Amit Dadheech, Deepak Sharma, Suresh Rajoriya and Garima Sharma. 2019. ISSR-Based Molecular Characterization of Opium poppy (Papaver somniferum L.) Genotypes.Int.J.Curr.Microbiol.App.Sci. 8(9): 2540-2545. doi: https://doi.org/10.20546/ijcmas.2019.809.294