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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2519-2529
DOI: https://doi.org/10.20546/ijcmas.2019.809.292


Growth of Trichoderma spp on Different Solid Substrates
B. Boblina*, S. K. Beura, M. K. Mishra and A. G. Panda
Department of Plant Pathology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha-751003, India
*Corresponding author
Abstract:

Trichoderma is one of the common fungal biocontrol agents being used worldwide for efficient management of various foliar and soil borne plant pathogens. Unlike chemicals, the establishment of these biocontrol agents in the targeted niche requires support even after their application. Two Trichoderma species (Isolate 2 and Isolate 5) were isolated from paddy and groundnut rhizospheres respectively. These two isolates were the two most vigorous ones among all the seven Trichoderma isolates obtained from different crop rhizospheres on the basis of their growth in different media and their inhibitory effects on two potential soil borne pathogens namely Rhizoctonia solani and Sclerotium rolfsii as observed in dual culture experiment. Present study deals with evaluation of eighteen solid substrates. Colony forming units were counted following the serial dilution technique and a gradual decrease was observed over a period of six months. Among different solid substrates tested, vermicompost was observed to be the best solid substrate for isolate 2 recording the maximum cfu count 463.67 x 107cfu/g of substrate in the first month after inoculation and it retained spore viability even after six months of inoculation giving a fairly good cfu count of 97.33 x107cfu/g. For isolate 5, groundnut shell performed the best, recording the maximum cfu count of 467.67 x 107 cfu/g in the first month and 144.00 x 107 cfu/g was retained even in the sixth month after inoculation.


Keywords: Rhizoctonia solani, ecosystem, disease management
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How to cite this article:

Boblina, B., S. K. Beura, M. K. Mishra and Panda, A. G. 2019. Growth of Trichoderma spp on Different Solid Substrates.Int.J.Curr.Microbiol.App.Sci. 8(9): 2519-2529. doi: https://doi.org/10.20546/ijcmas.2019.809.292