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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2060-2081
DOI: https://doi.org/10.20546/ijcmas.2019.809.238


Optimization, Purification and Characterization of Phytase from Isolated Probiotic Pediococcus acidilactici BNS5B
Bhawna Sharma and Geeta Shukla*
Department of Microbiology, Panjab University, Chandigarh-160014, India
*Corresponding author
Abstract:

Microbial phytases are of immense importance due to their application in food/feed industry by enhancing the availability of essential minerals such as phosphorous, iron, calcium etc. required for normal human physiology and also have commercial and environment significance. Therefore, in present study an attempt was made to enhance the production of phytase from isolated probiotic Pediococcus acidilactici BNS5B by employing both one variable at a time approach and statistically based design of experiments such as Plackett-Burman and Response Surface Methodology. Interestingly, phytase production was enhanced 94 fold at an optimised condition of 0.8% galactose, 1.25% yeast extract, 1.25% beef extract and 1.25% ammonium sulphate. Further, the phytase enzyme was purified and had apparent molecular weight of 43 KDa, pH optima of 5.5 with pH stability in the range of 2.5-6.5, temperature optima of 40°C and retaining an activity of 76% at a temperature range of 20-80°C and followed normal Michael-Menten curve with the kinetic parameter Km and Vmax of 0.5455mM and 33.927µmol/min respectively. Taken together, it is suggested that phytase from probiotic Pediococcus acidilactici BNS5B can be employed to enhance mineral bioavailability in food/feed industry, but needs to be correlated both experimentally and clinically.


Keywords: Plackett-Burman Design, Response Surface Methodology, Ion-exchange chromatography
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How to cite this article:

Bhawna Sharma and Geeta Shukla 2019. Optimisation, Purification and Characterisation of Phytase from Isolated Probiotic Pediococcus acidilactici BNS5B.Int.J.Curr.Microbiol.App.Sci. 8(9): 2060-2081. doi: https://doi.org/10.20546/ijcmas.2019.809.238