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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1644-1657
DOI: https://doi.org/10.20546/ijcmas.2019.809.187


Characterization of Recombinant Bean Yellow Mosaic Virus belongs to Group-IV Infecting Gladiolus
P. Hemachandra Reddy1, 2, V. Venkataravanappa3, P. Swarnalatha1, K. V. Ashwathappa1 and M. Krishna Reddy1*
1Division of Plant Pathology, Indian Institute of Horticultural Research (IIHR), Bangalore, India
2Department of Biotechnology, Centre for Post-Graduate Studies, Jain University, Bangalore, India
3Division of Plant Pathology, Central Horticultural Experiment Station, Chettali, India
*Corresponding author
Abstract:

Gladiolus is an important vegetatively propagated ornamental plant cultivated in different parts of the world. The major constrains for production quality and quantity of flowers in gladiolus is due to infection of different viruses. During survey, the gladiolus plants (Ten samples) with mosaic symptoms were collected from different farmer fields in Bangalore rural district and were confirmed for Bean yellow mosaic virus (BYMV) infection by ELISA and PCR using specific primers. Further compete genome of BYMV that infects gladiolus was amplified cloned and sequenced. The analysis showed that the genome of BYMV shared maximum nucleotide identity 92-97.2% with BYMV isolates belonging to the group IV infecting different crops in India, Japan, USA and Taiwan. Further recombination analysis showed that most part of the genome was derived from BYMV isolates from the phylogenic group of IV and I to emerge as a new variant of BYMV infecting gladiolus. The significance of these findings is discussed.


Keywords: Bean yellow mosaic virus, Gladiolus,Phylogenetic analysis, RT-PCR and Recombination.
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How to cite this article:

Hemachandra Reddy, P., V. Venkataravanappa, P. Swarnalatha, K. V. Ashwathappa and Krishna Reddy, M. 2019. Characterization of Recombinant Bean Yellow Mosaic Virus belongs to Group-IV Infecting Gladiolus.Int.J.Curr.Microbiol.App.Sci. 8(9): 1644-1657. doi: https://doi.org/10.20546/ijcmas.2019.809.187