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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1611-1624
DOI: https://doi.org/10.20546/ijcmas.2019.809.184


ISSR Analysis of genetic diversity in Acrocarpus fraxinifolius from three landscape elements of transition forest belt of Kodagu district, Karnataka, India
V. Maheswarappa1*, R. Vasudeva2, Ramakrishna Hegde3 and G. Ravikanth4 
1College of Forestry, Ponnampet, Kodagu- 571216, Karnataka, India
2Department of Forest Biology and Tree Improvement, College of Forestry, Sirsi-581401, India
3College of Forestry, Ponnampet-571216, India
4ATREE, Bengaluru, India
*Corresponding author
Abstract:

Acrocarpus fraxinifolius is an important tropical timber species mainly found in Asia and is a fast growing tree species found naturally in India, Chaina, Burma and Sumatra. In Karnataka, the species is extensively cultivated in coffee plantations due to its desirability in the rainy season that favours coffee growth. The species is also to the smaller extent noticed in natural forests and sacred groves of Kodagu district, Karnataka, India. However there was no much studies were taken in assessing the genetic diversity of the species exists in natural forests, sacred groves and coffee plantations. Hence the study was undertaken to know the extent of genetic diversity in the species as comparing to natural forests, sacred groves and coffee plantations was analyzed using ISSR markers. The leaf samples were collected from each of landscape. DNA was extracted from leaf material using Cetyl Trimethyl Ammonium Bromide (CTAB) technique. A total of 24 ISSR markers were used for this study, but only 14 ISSR primers were successfully amplified for 8 samples. Sampled populations from all the three landscapes showed relatively higher diversity. While the sacred grove and coffee plantations populations recorded higher diversity (0.3779 and 0.5601: 0.3661 and 0.5403, respectively) than natural forest population (0.2982 and 0.4567). This data clearly suggests that the farmers have conserved a higher level of diversity.


Keywords: Acrocarpus fraxinifolius, genetic diversity, ISSR, landscapes
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How to cite this article:

Maheswarappa, V., R. Vasudeva, Ramakrishna Hegde and Ravikanth, G. 2019. ISSR Analysis of genetic diversity in Acrocarpus fraxinifolius from three landscape elements of transition forest belt of Kodagu district, Karnataka, India.Int.J.Curr.Microbiol.App.Sci. 8(9): 1611-1624. doi: https://doi.org/10.20546/ijcmas.2019.809.184