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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
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Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1535-1549
DOI: https://doi.org/10.20546/ijcmas.2019.809.176


Genetic Divergence in Groundnut (Arachis hypogaea L.) using RAPD
Yaikhom Vivekananda1*, Pramesh Khoyumthem2, Mutum Suraj Singh3,  Konsam Cha Shyamananda3 and N. Brajendra Singh1
1Department of Plant Breeding and Genetics, College of Agriculture, Central Agricultural University, Imphal-795004, India
2AICRP(Groundnut), Central Agricultural University, India
3Farmer FIRST, Imphal Centre, Central Agricultural University, India
*Corresponding author
Abstract:

Twenty four genotypes of Arachis hypogaea (L.), of which 12 genotypes belonging to Virginia and 12 belonging to Spanish varieties were used to study the genetic divergence within its botanical varieties using RAPD. 16 primers belonging to OPH were used in the study. Out of the 16 primers utilized, 36 and 37 bands were produced in Virginia and Spanish group, respectively. Out of the total bands produced, 18 and 20 bands were polymorphic for Virginia and Spanish group, respectively. Jaccard’s similarity coefficient for Virginia group ranged from 0.09 to 0.78 and for Spanish group, it ranged from 0.13 to 0.88. A dendrogram was constructed using the similarity matrix value as determined from RAPD data for 24 groundnut genotypes. From the similarity coefficient it was found that the genotypes HNG 137 and ICGS 76 (0.09); HNG 137 and ICGV 87846 (0.09) showed maximum diversity among all genotypes for the Virginia group whereas the genotypes JSP 48 and K 1451 (0.78); K 1451 and K 1468 (0.78) showed the maximum similarity. Similarly, for the Virginia group the genotypes CSMG 2006-6 and J 71 (0.13); CSMG 2006-6 and RTNG 1 (0.13); K 1470 and RTNG 1 (0.013); J 71 and K 1470 showed maximum diversity whereas Dh 218 and K 1392 (0.88) showed the maximum similarity. The dendrogram clearly divided 12 genotypes of Virginia and Spanish groundnut genotypes into 4 and 5 clusters, respectively. The genetic relationships estimated can be useful for hybridization in the future groundnut improvement programme.


Keywords: RAPD, Groundnut, Genetic Divergence
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How to cite this article:

Yaikhom Vivekananda, Pramesh Khoyumthem, Mutum Suraj Singh, Konsam Cha Shyamananda and Brajendra Singh, N. 2019. Genetic Divergence in Groundnut (Arachis hypogaea L.) using RAPD.Int.J.Curr.Microbiol.App.Sci. 8(9): 1535-1549. doi: https://doi.org/10.20546/ijcmas.2019.809.176