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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1502-1508
DOI: https://doi.org/10.20546/ijcmas.2019.809.172


Characterization and Antibiotic Sensitivity Pattern of Nonfermenting Gram Negative Bacilli from various Clinical Samples at a Tertiary Care Hospital
Narayan Shrihari and M. Iswarya*
Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Cantonment area, Ballari-583104, Karnataka, India
*Corresponding author
Abstract:

Non-fermenting Gram negative bacilli (NFGNB) are a group of bacteria which are aerobic, non sporing and most commonly they are saprophytes. They are also found as commensals in both man and animals. NFGNB are previously considered as a contaminant but now it is emerged as a important cause for life threatening nosocomial infections and also multidrug resistant organisms. Materials and methods: One year prospective study was conducted and our aim is to characterise the non fermenters from various clinical samples and their antibiogram. 110 isolates from various clinical samples with different age groups were included. The clinical samples like pus, urine, endotracheal aspirates, blood, sputum and body fluids were collected under aseptic precaution and identified by using standard protocol. It includes Gram’s staining, Motility Testing, Catalase test, Oxidase test, OF test and various biochemical reactions. The susceptibility testing was done by Kirby bauer disc diffusion method. A total of 110 NFGNB were isolated and among that nonfermenters, Pseudomonas aeruginosa (49%) was the predominant, followed by Acinetobacter baumanii (35%), Acinetobacter lwoffi (7.3%), Stenotrophomonas maltophilia (5.4%), and Burkholderia cepacia (2.8%). Pseudomonas aeruginosa showed good sensitivity to Polymyxin B (100%), Meropenem and Imipenam (79.6%) followed by Piperacillin tazobactam (72%) and Amikacin (59.3%). Acinetobacter baumanii showed 92% sensitivity to Polymyxin B followed by 69% sensitivity to Meropenem and Imipenam. P.aeruginosa and A.baumanii were the commonest nonfermenters that are isolated in our study. They are associated with various infections like urinary tract infection, blood stream infections, surgical site infections and ventilator associated pneumonia. P.aeruginosa showed better sensitivity to following antibiotics: Polymyxin B, Meropenem, Imipenem and Amikacin. Therefore it is essential to identify the nonfermenters and to know their antibiotic sensitivity pattern.


Keywords: Non-Fermenting Gram negative Bacilli, Antibiotic sensitivity, Pseudomonas aeurginosa, Polymyxin B, Meropenem.
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How to cite this article:

Narayan Shrihari and Iswarya M 2019. Characterization and Antibiotic Sensitivity Pattern of Nonfermenting Gram Negative Bacilli from various Clinical Samples at a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 8(9): 1502-1508. doi: https://doi.org/10.20546/ijcmas.2019.809.172