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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1302-1311
DOI: https://doi.org/10.20546/ijcmas.2019.809.149


Isolation and Characterization of Exopolysaccharide producing Bacillus cereus from Brown Seaweed- Sargassum wightii
V. A. Minimol*, Pankaj Kishore, Ranjit K. Nadella, K. R. Sreelakshmi, S. S. Greeshma, M. M. Prasad and Suseela Mathew
ICAR-Central Institute of Fisheries Technology, Matsyapuri, P. O., Willingdon Island, Cochin, India
*Corresponding author
Abstract:

The bacterial extracellular polymeric substances (EPS) have huge applications in biotechnological and industrial sector. Biochemical composition determines its role in biofilm formation and pathogenicity as well as beneficial applications in various industries as thickeners, stabilizers, gelling agents etc. In the present study, aimed at screening the EPS producing bacteria from three species of brown seaweed and the EPS producing bacteria was isolated from Sargassium wightii. The EPS extraction was optimized and maximum production was recorded in BHI broth (1.65 mg ml-1) and media supplemented with glucose (1.56 mg ml-1). The extracted EPS contained 69.9% carbohydrate. Structural analysis by FTIR revealed the presence of carbohydrate (peak at 3292.93 cm−1, & 1200-1000 cm−1) and S-S stretch (peak at 600 cm−1 & 492 cm−1). The isolate was identified as Bacillus cereus by 16S rRNA sequencing and species specific PCR targeting bla gene. Complete study on biochemical composition and structure of EPS will facilitate the employment of this novel biopolymer in food and pharmaceutical industry.


Keywords: Exopolysaccharides, brown seaweeds, Bacillus cereus, FTIR
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How to cite this article:

Minimol, V. A., Pankaj Kishore, Ranjit K. Nadella, K. R. Sreelakshmi, S. S. Greeshma, M. M. Prasad and Suseela Mathew 2019. Isolation and Characterization of Exopolysaccharide producing Bacillus cereus from Brown Seaweed-Sargassum wightii.Int.J.Curr.Microbiol.App.Sci. 8(9): 1302-1311. doi: https://doi.org/10.20546/ijcmas.2019.809.149