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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1220-1231
DOI: https://doi.org/10.20546/ijcmas.2019.809.140


Genic Microsatellite Markers for Genetic Diversity in Wheat Genotypes
Manisha Kumari, Mukesh Kumar, Vikram Singh, S. Vijay Kumar* and Lakshmi Chaudhary
Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, India
*Corresponding author
Abstract:

Genetic diversity assessment is necessary to help tackle the threats of environmental fluctuations and for the effective exploitation of genetic resources in breeding program. Recent advancement in the field of molecular markers has made the genetic characterization of genotypes rapid, reliable and reproducible. In the present investigation, we have characterized 49 wheat genotypes at molecular level using 52 SSR primers (including Yr specific primers). 27 polymorphic SSR markers were dispersed over the AABBDD wheat genome, a total of 102 alleles were detected with allele range of 1 to 6. Polymorphism information content (PIC) values calculated to assess the informativeness of each marker ranged from 0.11 to 0.95 and there is significant that 5 out of 27 SSR loci, namely Xpsp 3000, Xwgp249, Wmc198, csLV34, Xgwm301 revealed PIC values above 0.70, can be considered highly useful for differentiation of wheat genotypes. The UPGMA cluster tree analysis led to the grouping of 49 wheat genotypes in two major clusters and nine sub clusters. Cluster pattern revealed that, sub-cluster six was the largest consisting maximum number of twelve genotypes. Our results suggested that the classification based on genotypic markers of these wheat genotypes would be useful for selection of varieties for wheat improvement program.


Keywords: Diversity, polymorphism, Simple Sequence Repeats, Yellow rust, Wheat
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How to cite this article:

Manisha Kumari, Mukesh Kumar, Vikram Singh, Vijay Kumar S and Lakshmi Chaudhary 2019. Genic Microsatellite Markers for Genetic Diversity in Wheat Genotypes.Int.J.Curr.Microbiol.App.Sci. 8(9): 1220-1231. doi: https://doi.org/10.20546/ijcmas.2019.809.140