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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 868-878
DOI: https://doi.org/10.20546/ijcmas.2019.809.104


Comparison of the Use of Three Different Stool Preservatives on the Morphology of Intestinal Parasites
P.P. Maneesha, Nonika Rajkumari*, R. Sneha, Shashiraja Padukone and Ajay Philips Selvaratthinam
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry-605006, India
*Corresponding author
Abstract:

Stool is one of the most difficult laboratory samples to be handled especially for recovery of intestinal parasites. The preservation of stool parasites faces a lot of difficulties in laboratory and the use of powerful preservatives leads to a better result. This study describes the efficacy of three stool preservatives for the stool parasites and they are 10% formalin, low viscosity polyvinyl alcohol and sodium acetate acetic acid formalin and compare them using fresh stool which were positive for intestinal parasites. Stool samples positive for an intestinal parasite by microscopy were taken and the outcome was compared after preserving the sample with each of the 3 preservatives and observed after 1 month period in relation to their morphology and staining properties. Of all the 3 preservatives, morphologic identification was more satisfactory with 10% formalin and least with low viscosity polyvinyl alcohol. However, all didn’t give much satisfying results with a permanent stain like trichrome though the best result both in terms of morphological identification and proper taking up of the stain was seen in those preserved with 10% formalin followed by SAF and least in those with LV-PVA. Using stool preservative is another viable alternative to using fresh stool and it helps to retain the morphology to a certain extent.


Keywords: Preservative, Parasite morphology, Formalin, Low viscosity poly vinyl alcohol, Sodium acetate-acetic acid –formalin
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How to cite this article:

Maneesha, P.P., Nonika Rajkumari, R. Sneha, Shashiraja Padukone and Ajay Philips Selvaratthinam. 2019. Comparison of the Use of Three Different Stool Preservatives on the Morphology of Intestinal Parasites.Int.J.Curr.Microbiol.App.Sci. 8(9): 868-878. doi: https://doi.org/10.20546/ijcmas.2019.809.104