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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 851-860
DOI: https://doi.org/10.20546/ijcmas.2019.809.102


In vitro Evaluation of Botanical Extracts, Animal Wastes, Organic and Inorganic Salts against Sclerotinia Rot of Rapeseed-Mustard Caused by Sclerotinia sclerotiorum (Lib) de Bary
P. Upadhyay* and A.K. Tiwari
Centre of advanced studies, Department of Plant Pathology, College of agriculture, GBPUA&T Pantnagar- 263145, U.S. Nagar, Uttarakhand, India
*Corresponding author
Abstract:

Among various diseases of rapeseed mustard, Sclerotinia rot caused by Sclerotinia sclerotiorum is emerging out as a serious problem and a major constraint for production and productivity of this crop across the country. Due to increasing environmental concern and health hazard the old management solution of this disease in form of fungicides, need to be replaced with safe and eco-friendly management approach. So the present experiment was carried out for in vitro evaluation of botanical extracts, animal wastes products and organic and inorganic salts against the growth of pathogen. Among them botanical extracts (garlic bulb, onion bulb, neem kernel, eucalyptus leaves) and animal waste products (cow urine, cow dung, vermin wash), were evaluated in vitro at different concentration levels (1, 2.5 and 10 %) by poison food technique while, organic and inorganic non toxic salts (sodium bicarbonate, oxalic acid, calcium carbonate and calcium sulphate) were evaluated at three concentration levels (0.2, 0.5, 1 and 2%) by same method for mycelium inhibition test. Among them cow urine at 5%, garlic bulb extract at 10% and sodium bi carbonate at 1% found effective with 100% inhibition of mycelial growth of the pathogen followed by cow dung at 10% concentration, calcium carbonate at 2% concentration with complete mycelia growth inhibition. Hence natural or eco-friendly products such as cow urin and garlic bulb extracts were found very effective in managing sclerotinia rot disease in vitro, which can further be evaluated under field conditions for confirmation to develop Integrated Disease Management (IDM) module for chemical free, safe and eco friendly management of Sclerotinia rot in future.


Keywords: Sclerotinia rot, Sclerotinia sclerotiorum, Rapeseed-Mustard, botanical extract, animal wastes, organic inorganic salts, management
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How to cite this article:

Upadhyay, P. and Tiwari, A.K. 2019. In vitro Evaluation of Botanical Extracts, Animal Wastes, Organic and Inorganic Salts against Sclerotinia Rot of Rapeseed-Mustard Caused by Sclerotinia sclerotiorum (Lib) de Bary.Int.J.Curr.Microbiol.App.Sci. 8(9): 851-860. doi: https://doi.org/10.20546/ijcmas.2019.809.102