Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 749-757
DOI: https://doi.org/10.20546/ijcmas.2019.809.090


Biological Indexing and Molecular Approaches in Detecting a Mild Strain ‘CRS 4’ against Citrus tristeza Virus in Khasi Mandarin (Citrus reticulata)
Borsha Rani Baruah1*, R. K. Kakoti2, A. Borbora2, S. Singh3, S. Saikia4 and P. D. Nath1
1Department of Plant Pathology,
3Department of Agricultural Biotechnology, Assam, India Agricultural University, Jorhat-13, India
 2Citrus Research Station, Tinsukia, 4Horticultural Research Station, Kahikuchi, Assam, India Agricultural University, Assam, India
*Corresponding author
Abstract:

Citrus tristeza virus, the most important viral disease of citrus, is reported in Assam and other NE states of India to infect Khasi mandarin (Citrus reticulata), the most economically important citrus crop of the region. For effective management, an attempt was made to identify a potential mild isolate against virus. Leaf samples were collected from Khasi Mandarin plants expressing differential symptoms from three different locations viz., Tinsukia, Golaghat and Mariani of Upper Brahmaputra Valley Zone of Assam. These were then grouped into three categories based on ELISA OD405 values. Biological indexing with CTV positive samples from these three serological categories on Mexican lime (Citrus aurantifolia) seedlings resulted in symptom expression within three months post grafting. Visible symptoms of CTV infection were observed in some of the graft successful indicator plants whereas, in Khasi mandarin selection ‘CRS 4’, no visible symptom development took place within this period. Based on the results, the plants were grouped into two groups- symptom producing and non-symptom producing, and were confirmed through Bi-directional RT-PCR with mild and severe strain primers. PCR products for ‘CRS 4’ were sequenced. Consensus sequences showed a single nucleotide difference at position 371 for mild isolates (CRS 4), thereby confirming the identity.


Keywords: Citrus tristeza virus, Khasi mandarin, mild strain, biological indexing, BD/RT-PCR, Assam
Download this article as Download

How to cite this article:

Borsha Rani Baruah, R. K. Kakoti, A. Borbora, S. Singh, S. Saikia and Nath, P. D. 2019. Biological Indexing and Molecular Approaches in Detecting a Mild Strain ‘CRS 4’ against Citrus tristeza Virus in Khasi Mandarin (Citrus reticulata)Int.J.Curr.Microbiol.App.Sci. 8(9): 749-757. doi: https://doi.org/10.20546/ijcmas.2019.809.090