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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 474-479
DOI: https://doi.org/10.20546/ijcmas.2019.809.057


Culture and Detection of NDV Virus by Haemagglutination Test (HA) and Haemagglutination Inhibition Test (HI)
Ojasvita1*, Sanjay Kapoor2, Ajit Singh3, Satbir Sharma4, Mahavir Singh2, Pankaj Kumarand Rajendra Yadav6
1Department of Animal Husbandary and Dairying, Haryana
2Department of Veterinary Microbiology (LUVAS, Hisar)
3Department of Veterinary Microbiology (LUVAS, Hisar)
4Department of Veterinary Surgery (LUVAS, Hisar)
5Disease investigation laboratory, Rohtak (LUVAS, Hisar)
6RVDEC, Mahendergarh (LUVAS, Hisar)
*Corresponding author
Abstract:

Present study on NDV was carried out at Department of Veterinary Microbiology, LUVAS, Hisar. As we all know that Newcastle disease is also known as Ranikhet disease in India. Virus causes a worldwide disease of birds e.g. chickens, turkeys, guinea fowl, pheasants and pigeons. Man is susceptible and suffers from self limiting conjunctivitis. It is transmitted by droplet, direct contact, fomites or the ingestion of excreted virus and its average incubation period is 5-6 days but it may vary from 2-15 days. In severe outbreaks the symptoms appear within 3 days. So efforts had been made to culture, detect and confirm NDV virus from 15 tissue samples. Only two out of 15 samples and La Sota vaccine strain could be confirmed to contain NDV on the basis of HA and HI tests.


Keywords: NDV, HA, HI and Titration
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How to cite this article:

Ojasvita, Sanjay Kapoor, Ajit Singh, Satbir Sharma, Mahavir Singh, Pankaj Kumar and Rajendra Yadav. 2019. Culture and Detection of NDV Virus by Haemagglutination Test (HA) and Haemagglutination Inhibition Test (HI).Int.J.Curr.Microbiol.App.Sci. 8(9): 474-479. doi: https://doi.org/10.20546/ijcmas.2019.809.057