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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 369-373
DOI: https://doi.org/10.20546/ijcmas.2019.809.044


Field Evaluation of Backcross Progenies of Improved Samba Mahsuri Possessing SCM2 Gene
P. Jayamma1, 2, C.H. Anuradha1, P. Senguttuvel2, E. Punniakotti2, M.B.V.N. Kousik2, G. Harika2, K. Chaitra2, T. Ramesh1, D. Subramanyam2 and R.M. Sundaram2*
1Institute of Biotechnology, Professor Jayashankar Telangana State Agricultural University (PJTSAU), Rajendranagar, Hyderabad 500030, India
2Indian Council of Agricultural Research (ICAR)-Indian Institute of Rice Research (IIRR), Rajendranagar, Hyderabad 500030, India
*Corresponding author
Abstract:

India ranks second in production of rice (Oryza sativa). The culm plays an important role in determining the lodging resistance and crop architecture of rice. The gene, STRONG CULM (SCM2) has been reported to be associated with strong culm. In the present study, BC2F2 lines in the background of Improved Samba Mahsuri (ISM) derived by crossing Improved Samba Mahsuri (ISM) and a breeding line of Swarna possessing SCM2 were genotyped for presence of SCM2 with a newly designed gene specific marker (SCM2-8F/8R) and plants positive for the gene were then phenotyped and measured for Culm thickness with an average of three plants per each line of the BC2F2 selected best lines along with the parents for culm thickness by measuring the Culm diameter with Vernier Calipers and by observing the Internode Culm cross sections under Microscope. All the backcross plants possessing the gene had significantly better culm thickness, and culm diameter as compared to Improved Samba Mahsuri indicating a perfect co-segregation of trait phenotype with marker genotype, validating the utility of the marker. The backcross plants of ISM possessing increased culm strength are being crossed with breeding lines of ISM possessing higher number of per panicle.


Keywords: Back cross introgression lines, Improved Samaba Mashuri, SCM2, Gene specific and allele specific marker, Codominant marker, Culm diameter, Millimeters and µm
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How to cite this article:

Jayamma, P., C.H. Anuradha, P. Senguttuvel, E. Punniakotti, M.B.V.N. Kousik, G. Harika, K.Chaitra, T. Ramesh, D. Subramanyam and Sundaram, R.M. 2019. Field Evaluation of Backcross Progenies of Improved Samba Mahsuri Possessing SCM2 Gene.Int.J.Curr.Microbiol.App.Sci. 8(9): 369-373. doi: https://doi.org/10.20546/ijcmas.2019.809.044