Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 3026-3031
DOI: https://doi.org/10.20546/ijcmas.2019.808.350


Detection of Infectious Bronchitis Virus from Chicken Manifesting Respiratory Signs by Reverse Transcriptase Polymerase Chain Reaction
Niranjana S. Rajalakshmi1, Surya Sankar1*, Anu Bosewell1, U. Rashi1, M. Mini1Binu K. Mani2 and I.S. Sajitha3
1Department of Veterinary Microbiology,
3Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India
2Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala, India
*Corresponding author
Abstract:

The Indian poultry industry in spite of exhibiting excellent performance in poultry production and rearing is being put to several challenges in various fronts, particularly in the form of production losses due to several diseases. Considering the reports on the occurrence of avian respiratory diseases suspected for infectious bronchitis (IB) from different parts of Kerala, in the present study, a reverse transcriptase polymerase chain reaction (RT - PCR) was employed for rapid detection of infectious bronchitis virus. Tissue samples from ailing birds of different age groups manifesting respiratory signs which were brought to the Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy during the period from June 2018 to June 2019 for disease investigation were subjected to ribonucleic acid extraction, complementary cDNA synthesis and subsequently RT - PCR. Among 87 samples, 37 were positive for IB.


Keywords: Infectious bronchitis, RT - PCR, Respiratory pathogens
Download this article as Download

How to cite this article:

Niranjana S. Rajalakshmi, Surya Sankar, Anu Bosewell, U. Rashi, M. Mini, Binu K. Mani and Sajitha, I.S. 2019. Detection of Infectious Bronchitis Virus from Chicken Manifesting Respiratory Signs by Reverse Transcriptase Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 8(8): 3026-3031. doi: https://doi.org/10.20546/ijcmas.2019.808.350