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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 2823-2835
DOI: https://doi.org/10.20546/ijcmas.2019.808.325


Condition Optimization for Phosphate Solubilization by Kosakonia cowanii MK834804, an Endophytic Bacterium Isolated from Aegle marmelos
Sangeeta Panigrahi1 and Chandi Charan Rath2*
1Department of Botany, College of Basic Science and Humanities, Odisha University of Agriculture and Technology (OUAT), Bhubaneswar, 751003, India
2Department of Life Sciences, Rama Devi Women’s University, Bhubaneswar, 751022, India
*Corresponding author
Abstract:

In the present study endophytic bacteria were isolated from Aegle marmelos and were screened for phosphate solubilizing activity. The isolate AP01 showed the ability to solubilize inorganic tricalcium phosphate and thus was characterized. Phenotypic, biochemical and genotypic analysis revealed, the isolate to be Kosakonia cowanii, a gram –ve proteobacterium of family enterobacteriaceae with accession no. MK834804, of the three media viz. Pikovskaya’s agar (PKVA), National Botanical Research Institute’s phosphate growth medium (NBRIP) and NBRIP-BPB (Bromo Phenol Blue) agar media tested for Phosphate solubilizing efficiency, the isolate showed high Phosphate solubilizing Index (PSI 4.5) in PKVA medium. Whereas, under submerged fermentation the isolate showed highest phosphate solubilization in NBRIP medium (70.200µg/ml) on 4th day with maximum drop down of medium pH (3.6). Optimum solubilization of phosphorus was observed at temperature 37ºC (70.403µg/ml), pH 8 (71.748 µg/ml) with 0.5 % ammonium sulphate (79.76µg/ml) and 2% Lactose (87.283µg/ml) on 4th day of incubation period as optimal nitrogen and carbon sources respectively. Release of various organic acids i.e. oxalic acid, malic acid, tartaric acid and gluconic acid into the medium was characterized by High Performance Liquid Chromatography (HPLC), during phosphate solubilization by the isolate.


Keywords: Phosphate solubilization. Aegle marmelos, Kosakonia cowanii, Tricalcium phosphate, Organic acid
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How to cite this article:

Sangeeta Panigrahi and Chandi Charan Rath. 2019. Condition Optimization for Phosphate Solubilization by Kosakonia cowanii MK834804, an Endophytic Bacterium Isolated from Aegle marmelos.Int.J.Curr.Microbiol.App.Sci. 8(8): 2823-2835. doi: https://doi.org/10.20546/ijcmas.2019.808.325