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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 386-396
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.043


Optimization of Cultural Conditions for Production of Antifungal Bioactive Metabolites by Streptomyces spp. Isolated from Soil
Charu Singh*, Ramendra Singh Parmar, Pragya Jadon and Ajay Kumar
Department of Life Sciences, ITM University, Gwalior (M.P), India
*Corresponding author
Abstract:

The present research was undertaken with an aim to isolate and characterize antifungal compounds from actinomycetes and optimization of cultural characteristics for production of bioactive metabolite by actinomycetes. Actinomycetes isolate ACITM-1 was further cultivated in different conditions fermentation in order to optimize biosynthetic process of antibiotic production including: different pH values, different temperatures, different carbon and nitrogen sources were fully investigates. Actinomycetes isolate ACITM-1 was isolated from soil of Gwalior Chambal region, MP, (India) was found to show significant antifungal activity against phytopathogens of soybean crop. Based on physiological, biochemical characteristics the isolate was identified as Streptomyces chilikensis ACITM-1. This strain was further cultivated in different conditions in order to optimize the growth of secondary metabolite production. The antimicrobial activity and biomass production was highest when isolates were cultivated in media having starch as a carbon source. Peptone and potassium nitrate were found best nitrogen source for the highest antifungal activity and biomass production. Form the experiment it was observed that antifungal activity was found maximum with starch, peptone and potassium nitrate in 96 hours at 30º C.


Keywords: Antibiotic, Antifungal activity, Optimization, Screening.
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How to cite this article:

Charu Singh, Ramendra Singh Parmar, Pragya Jadon and Ajay Kumar. 2017. Optimization of Cultural Conditions for Production of Antifungal Bioactive Metabolites by Streptomyces spp. Isolated from Soil.Int.J.Curr.Microbiol.App.Sci. 6(2): 386-396. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.043