Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1945-1956
DOI: https://doi.org/10.20546/ijcmas.2019.808.229


The Spontaneous Haemolytic Activity of Catla catla Blood Serum Represents the Characteristics Features
Pushpa Choudhary1, 2* and M. Makesh1, 3
1Fish Health Management Division, ICAR- Central Institute Freshwater Aquaculture, Bhubaneswar, 751002, Odisha, India
2Aquatic Environment and Health Management Division, Central Institute of Fisheries Education, Versova, Mumbai 400061, India
3Fin Fish Culture Division, ICAR-Central Institute of Brackishwater Aquaculture, Chennai 600028, India
*Corresponding author
Abstract:

In this study an attempt was taken to determine the spontaneous haemolytic activity 50 % (SH50%) of healthy catla (Catla catla) fingerlings serum with respect to its characteristics features such as optimal temperature, ability for lysing red blood cells (RBCs) from various animals, heat sensitivity, storage temperature and effect of different agents like EDTA, EGTA. In addition to these, complement binding factors such as Zymosan, Lipopolysacharide were also tested for SH 50% activity. Furthermore, the effect of bacterial culture was also determined on SH 50% activity of catla sera. The most advantageous temperature for rabbit RBCs lysis was determined as 20 °C for catla serum. The normal catla serum was found to be heat sensitive and it was obtained as more than 60 °C. The haemolytic activity of catla sera showed reasonable activity after short term storage at -20 °C and good activity after six months storage at -80 °C. Furthermore, the chemical effects of ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and Ethylenediaminetetra acetic acid (EDTA) on SH 50 % activity was found to be opposite in action, inhibition of SH 50 % was obtained with EGTA whereas enhanced SH 50% activity with EDTA. Also, the inhibitory effects of Zymosan, bacterial LPS and Aeromonas hydrophila culture were detected for haemolytic activity of catla sera.


Keywords: Complement, Spontaneous haemolytic activity, Heat sensitivity, Zymosan, A. hydrophila; Labeorohita
Download this article as Download

How to cite this article:

Pushpa Choudhary and Makesh, M. 2019. The Spontaneous Haemolytic Activity of Catla catla Blood Serum Represents the Characteristics Features.Int.J.Curr.Microbiol.App.Sci. 8(8): 1945-1956. doi: https://doi.org/10.20546/ijcmas.2019.808.229