Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1830-1845
DOI: https://doi.org/10.20546/ijcmas.2019.808.216


Cultural, Morphological, Physiological and Biochemical Characteristics of Magnaporthe grisea Isolates of Finger Millet
S. Shanmugapackiam1*, S. Irulandi3, P. Murali Sankar2, A. Nagaraja3 and T. Raguchander1
1ICAR – Krishi Vigyan Kendra, Vamban, Pudukkottai – 622 303, India
2Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641 003, India
3Department of Entomology, Tamil Nadu Agricultural University, Coimbatore – 625 601, India
4Project Co-ordinating Unit, AICSMIP, GKVK, Bangalore 560 065, India
*Corresponding author
Abstract:

A total of 24 blast infected samples were collected from major finger millet growing areas of Tamil Nadu and also from All India Coordinated Small Millets Improvement Project Centres (ICAR) in India. Pathogenicity test with Magnaporthe grisea isolates showed highly significant differences in virulence on the variety KM 252 in the severity of leaf blast. Among the five different media tested, the Oat Meal Agar medium (OMA) was found to be best for the growth of M. grisea isolates and all the isolates produced white colony colour. The mean mycelial growth was maximum in OMA medium with 80.12 mm. The temperature 25 0C and pH 6.5 was found to be ideal for growth, sporulation and mycelial dry weight of M. grisea isolates. Of the different carbon sources, Richard’s medium with supplemented dextrose recorded the maximum mean mycelial growth of M. grisea isolates with 83.74 mm. Among the nitrogen sources, the Richard’s medium supplemented with NaNO3 recorded the maximum mean mycelial growth of 85.87 mm. This study was conducted to isolate, identify and characterize the pathogen using cultural, morphological, physiological and biochemical methods.


Keywords: Finger millet, Blast disease, Magnaporthe grisea, Characterization
Download this article as Download

How to cite this article:

Shanmugapackiam, S., S. Irulandi, P. Murali Sankar, A. Nagaraja and Raguchander, T. 2019. Cultural, Morphological, Physiological and Biochemical Characteristics of Magnaporthe grisea Isolates of Finger Millet.Int.J.Curr.Microbiol.App.Sci. 8(8): 1830-1845. doi: https://doi.org/10.20546/ijcmas.2019.808.216