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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 2527-2543
DOI: https://doi.org/10.20546/ijcmas.2019.808.294


Differential DNA Methylation under Drought Stress in Maize
Nehal Sallam1,*, Mounir Moussa1, Mohamed Yacout 1 and Ayman El-Seedy1
Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, 21545, Egypt
*Corresponding author
Abstract:

DNA methylation plays a vital roles in plant response to drought stress. Therefore, the present study was carried out to investigate differential cytosines methylation in leaves of maize under drought stress by using AMP method. Thirty days old maize seedlings were subjected to water deficit for nine days and the leaves of drought stressed plants and control plants were collected and stored at -20 until DNA extraction and subsequence DNA analysis. We isolated and sequenced 18 differentially methylated fragments. DMF 4, DMF 10, and DMF 13 were found to be homologous to lncRNA genes, DMF3 is similar to protein dehydration-induced 19 homologue 3 and DMF 18 is homologous to nudix hydrolase 2 gene. The obtained results indicated that lncRNAs are consider as important regulators in response of maize plant to drought stress.


Keywords: DNA Methylation, Drought Stress, Maize, DNA analysis
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How to cite this article:

Nehal Sallam, Mounir Moussa, Mohamed Yacout and Ayman El-Seedy. 2019. Differential DNA Methylation under Drought Stress in Maize.Int.J.Curr.Microbiol.App.Sci. 8(8): 2527-2543. doi: https://doi.org/10.20546/ijcmas.2019.808.294