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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1669-1674
DOI: https://doi.org/10.20546/ijcmas.2019.808.197


Electrophoretic Profile of Seminal Plasma Proteins of Specific Fractions of Large White Yorkshire Boar Semen
Malati Naik1*, Hiron M. Harshan1, K. Promod1, C.P. Abdul Azeez1, A.P. Usha2, C. Sunanda3 and C.R. Vishak2
1College of Veterinary and Animal Sciences, Pookode, Lakkidi P.O., Wayanad, Kerala – 673 576, India
2Director of Farms, KVASU and Professor and Head, Centre for Pig Production and Research, Mannuthy, India
3Department of Statistics, CVAS, Pookode, India
*Corresponding author
Abstract:

The objective of the research was to study the protein content and electrophoretic profile of specific fractions of Large White Yorkshire (LWY) boar semen. Sperm rich fractions (SRF) of semen were collected from four sexually mature LWY boars as fractions of 10 mL each, by gloved hand method. For analysis, the first 10 mL of the SRF, rest of the SRF and sperm free fraction (SFF) were collected separately. The protein content of the semen fractions was assessed and the proteins were subjected to one dimensional discontinuous sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to assess the electrophoretic profile. A protein content of 20.71 ± 2.63 mg/mL was observed in the first 10 mL of SRF, 21.77 ± 1.84 mg/mL in the remaining SRF and 20.52 ± 2.06 mg/mL in the SFF. An average number of 13.5 ± 0.51 bands were observed in first 10 mL of SRF, 12.92 ± 0.44 bands in the remaining part of SRF and 12.78 ± 0.23 bands in the SFF. The proteins observed varied in the molecular weight from 18 - 244 kDa, 8 – 242 kDa and 9 – 248 kDa in first 10 mL of SRF, remaining SRF and SFF, respectively.


Keywords: Boar semen, SDS-PAGE, Seminal plasma protein, Sperm rich fraction
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How to cite this article:

Malati Naik, Hiron M. Harshan, K. Promod, C.P. Abdul Azeez, A.P. Usha, C. Sunanda and Vishak, C.R. 2019. Electrophoretic Profile of Seminal Plasma Proteins of Specific Fractions of Large White Yorkshire Boar Semen.Int.J.Curr.Microbiol.App.Sci. 8(8): 1669-1674. doi: https://doi.org/10.20546/ijcmas.2019.808.197