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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1747-1753
DOI: https://doi.org/10.20546/ijcmas.2019.808.206


Bacteriological Profile of the Organisms Causing Septicaemia and their Antibiotic Resistance Patterns
V. Deepali and R. Shyamala*
Department of Microbiology, MRMCW, Hyderabad, India
*Corresponding author
Abstract:

Blood stream infections are mainly caused by multi drug resistant organisms which more likely prolong the hospital stay and leads to increased health care costs. Hence, early diagnosis and treatment with appropriate antibiotics is the only way to treat bacteraemia. To study the bacteriological profile of the organisms causing septicaemia and their antibiotic resistance patterns to guide the clinicians to initiate the empirical therapy. Blood samples were collected aseptically from patients before taking any antibiotics. Samples were inoculated into Brain heart infusion broth and incubated at 37 degree for 24 hours. Blind subculture was done on to fresh 5% sheep blood agar and MacConkey agar. A negative result was followed up by examining the broth daily and doing a final subculture at the end of 7th day organisms identified by colony morphology and standard biochemical tests. A total of 273 blood samples were analysed, out of which 50 yielded growths (18.3%). Among this, 49 (98%) yielded bacterial isolates and 1 (2%) was Candida species. Out of 49 bacterial isolates, 28 (56%) were Gram positive organisms and 21 (42%) were Gram negative organisms. Among Gram positive isolates Coagulase negative Staphylococci (36%) were the predominant isolates. Among the Gram negative organisms, Escherichia coli (22%) were the predominant isolate. The incidence of blood stream infection was 18.3%. Majority were bacterial isolates. Gram positive were more than gram negative. Among Gram positive isolates. Coagulase negative Staphylococci (36%) were the predominant isolates. Among the Gram negative organisms, Escherichia coli (22%) was the predominant isolate


Keywords: Bacteriological profile, Organisms, septicaemia, Antibiotic resistance patterns, Therapy
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How to cite this article:

Deepali, V. and Shyamala, R. 2019. Bacteriological Profile of the Organisms Causing Septicaemia and their Antibiotic Resistance Patterns.Int.J.Curr.Microbiol.App.Sci. 8(8): 1747-1753. doi: https://doi.org/10.20546/ijcmas.2019.808.206