Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1535-1541
DOI: https://doi.org/10.20546/ijcmas.2019.808.180


Association Study of Single Nucleotide Polymorphism in Exon 3 of SPAG11B Gene with Conception Rate in Murrah Bulls
Bharti Deshmukh1*, Archana Verma2, Ishwar Dayal Gupta2, Neeraj Kashyap3 and Rajalaxmi Behera4
1Department of Veterinary and Animal Husbandry Extension Education, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
2Division of Animal Genetics and Breeding, ICAR-National Dairy Research Institute, Karnal, India
3Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
4ICAR-National Dairy Research Institute, Eastern Research Station, Kalyani, Kolkata, India
*Corresponding author
Abstract:

Present study was conducted with the objectives to identify single nucleotide polymorphism in sperm associated antigen 11 B(SPAG11B) gene and to analyze association between identified polymorphism with conception rate in Murrah bulls in ICAR-National Dairy Research Institute (NDRI) herd, Karnal. A 373 base pair region covering partial intron 2, exon 3 and partial intron3 of bovine SPAG11 gene was amplified using genomic DNA extracted from eighty six Murrah bulls and genotyped using sequencing and polymerase chain reaction- restriction fragment length polymorphisms (PCR-RFLP) methods. Alignment of edited sequence of Murrah buffalo with reported Bos taurus sequence (AC_000158.1) was done with ClustalW analysis. Gene and genotype frequencies, effective allele number, average heterozygosity and polymorphic information content of different genotypes were estimated by POPGENE version 1.32 (University of Alberta, Canada). Statistical analysis for Conception rate of Murrah bulls under study was carried out by SAS Version 9.3(SAS Institute Inc., Cary, NC, USA). The 373 bp fragment of SPAG11 gene was amplified using PCR and a novel SNP of G to A substitution at 2266 base of the SPAG11B gene was identified by sequencing. The amplicon was further digested with SNP-specific MunI restriction enzyme that showed three distinct genotypes viz., AA (266 bp and 107 bp fragment), GA (307 bp, 266 bp and 107 bp) and GG (373 bp fragment). Least square means of conception rate for the SNP was estimated and compared after correction for non-genetic factors. The identified novel SNP of SPAG11 gene showed non-significant association with conception rate. However, there is a lack of association, further studies have to be undertaken in a large population in order to validate the impact of g.2266G>A on conception rate in Murrah bulls.


Keywords: SPAG11B, Murrah bulls, PCR-RFLP, Conception rate
Download this article as Download

How to cite this article:

Bharti Deshmukh, Archana Verma, Ishwar Dayal Gupta, Neeraj Kashyap and Rajalaxmi Behera. 2019. Association Study of Single Nucleotide Polymorphism in Exon 3 of SPAG11B Gene with Conception Rate in Murrah Bulls.Int.J.Curr.Microbiol.App.Sci. 8(8): 1535-1541. doi: https://doi.org/10.20546/ijcmas.2019.808.180