National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Staphylococcus aureus is a main cause of bovine mastitis and a major pathogen affecting human health. The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) has become a significant concern for both animal health and public health. This study investigated the incidence of MRSA in milk samples collected from bovine mastitis and characterised the MRSA isolates using genotypic methods. A total of 55 Staphylococcus aureus isolates were identified from bovine mastitis by phenotypic methods such as colony morphology, Gram’s staining and biochemical tests. Genotypic confirmation was made by using Sa442 gene based PCR. For the phenotypic detection of methicillin resistant Staphylococcus, CHROM agar was found superior to the oxacillin screen agar. This provides an alternative for the detection of MRSA in clinical laboratories, especially when PCR is unavailable. Out of 11 MRSA isolates, only 4 isolates were found to be positive for methicillin resistance targeting a 533 bp fragment, which has led the scientists to think about possible mechanism rendering Staphylococcus resistant to beta lactamase other than presence of mecA gene. In the phylogenetic analysis, mecA gene sequence of all the four MRSA showed maximum nucleotide identity with the Human MRSA isolates which indicated that cross species spilling of MRSA had rendered it as one of the important zoonotic bacteria.
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