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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 223-230
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.029


Identification and Characterisation of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Mastitis
A.G. Bhagat*, H.N. Kher, A.I. Dadawala, H.C. Chauhan, B.K. Patel, M.D. Shrimali and B.S. Chandel
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University,Sardarkrushinagar- 385 506, Gujarat, India
*Corresponding author
Abstract:

Staphylococcus aureus is a main cause of bovine mastitis and a major pathogen affecting human health. The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) has become a significant concern for both animal health and public health. This study investigated the incidence of MRSA in milk samples collected from bovine mastitis and characterised the MRSA isolates using genotypic methods. A total of 55 Staphylococcus aureus isolates were identified from bovine mastitis by phenotypic methods such as colony morphology, Gram’s staining and biochemical tests. Genotypic confirmation was made by using Sa442 gene based PCR. For the phenotypic detection of methicillin resistant Staphylococcus, CHROM agar was found superior to the oxacillin screen agar. This provides an alternative for the detection of MRSA in clinical laboratories, especially when PCR is unavailable. Out of 11 MRSA isolates, only 4 isolates were found to be positive for methicillin resistance targeting a 533 bp fragment, which has led the scientists to think about possible mechanism rendering Staphylococcus resistant to beta lactamase other than presence of mecA gene. In the phylogenetic analysis, mecA gene sequence of all the four MRSA showed maximum nucleotide identity with the Human MRSA isolates which indicated that cross species spilling of MRSA had rendered it as one of the important zoonotic bacteria.


Keywords: Bovine mastitis, MRSA,PCR and Sequencing.
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How to cite this article:

Bhagat, A.G., H.N. Kher, A.I. Dadawala, H.C. Chauhan and Chandel, B.S. 2017. Identification and Characterisation of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Mastitis.Int.J.Curr.Microbiol.App.Sci. 6(2): 223-230. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.029