Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1293-1306
DOI: https://doi.org/10.20546/ijcmas.2019.808.153


DNA Barcoding on Cardinalfishes (Apogonidae) of Thoothukudi Coast
R. Rajeshkannan1*, J. Jaculine Pereira2, K. Karal Marx3, P. Jawahar2, D. Kiruthiga Lakshmi2 and Devivaraprasad Reddy4
1Dr. M.G.R. Fisheries College and Research Institute, Ponneri – 601204, India
2Fisheries College and Research Institute, Thoothukudi – 628008, India
3Institute of Fisheries Post Graduate Studies, OMR Campus, Vanniyanchavadi–603103, India
4Fisheries, Y.S.R. Horticulture University, Andhra Pradesh, India
*Corresponding author
Abstract:

Cardinalfishes belongs to the family, Apogonidae is cryptic in nature that often shows taxonomic ambiguity through conventional taxonomy. It is globally accepted that mitochondrial DNA marker i.e., Cytochrome C Oxidase (COI) can be used to resolve these taxonomic uncertainties. In the present study, the DNA barcode was developed using COI marker for the two species of cardinalfishes (Archamia bleekeri and Ostorhinchus fleurieu) collected from Thoothukudi coast. Results showed that the distance values between the two species are higher than that of within the species. The Cytochrome C Oxidase subunit I (COI) gene showed more number of transitional pairs (Si) than transversional pairs (Sv) with a ratio of 2.4. The average distance values between A. bleekeri and O. fleurieu were 3.825, 4.704, 5.145, 7.390, 8.148, 7.187 and distance values among the A. bleekeri and O. fleurieu were 4.777 and 3.660, 3.583 and 6.509 respectively using K2P parameter. The average nucleotide frequency calculated were A= 26.9%, T(U)= 24.3%, C= 21.95% and G= 26.8%. The estimated GC content of A. bleekeri and O. fleurieu was (49.9%) and (47.6%) respectively and the average GC content was found to be 48.75%. Phylogenic trees were constructed individually for the two species using MEGA 6.0 software and the Neighbour-Joining tree showed distinct clusters shared by the species of same genera. In conclusion, the present study developed DNA barcode database for the two species of cardinal fishes that can be used for taxonomic purposes for these species.


Keywords: Apogonids, DNA barcoding, Cardinalfishes, Gulf of Mannar, Tuticorin, Conservation
Download this article as Download

How to cite this article:

Rajeshkannan, R., J. Jaculine Pereira, K. Karal Marx, P. Jawahar, D. Kiruthiga Lakshmi and Devivaraprasad Reddy. 2019. DNA Barcoding on Cardinalfishes (Apogonidae) of Thoothukudi Coast.Int.J.Curr.Microbiol.App.Sci. 8(8): 1293-1306. doi: https://doi.org/10.20546/ijcmas.2019.808.153