Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 165-173
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.014


Molecular Study of Clarithromycin Resistant Helicobacter pylori strains from Egyptian Centre
Maysaa El Sayed Zaki, Doaa M. Sherif, Mahmoud Abdelwahab Ali, Ahmed Shehta, Ahmed Megahed, Mona Abdel Latif Alsayed, Tarek Barakat and Sheren M Elzeny
Clinical Pathology Department, Children Hospital, Mansoura University, Egypt
Microbiology and Immunology Department, Children Hospital, Mansoura University, Egypt
Gastroenterology Surgical Center, Children Hospital, Mansoura University, Egypt
Gastroenterology unit, Children Hospital, Mansoura University, Egypt
*Corresponding author
Abstract:

Helicobacter pylori (H.pylori) is a common infection in Egypt. However, there are few data available about its antibiotics susceptibility. The aim of the present study was to detect to the presence of Primary clarithromycin resistance among H.pylori isolated from gastric biopsies from children and adults by the use of PCR-RFLP technique for detection of 23S rRNA gene mutations. The study included seventy four adults patients and thirty four children refereeing for Gastroenterology centre and Mansoura University Children hospital, Egypt from December 2014 till August 2015. Endoscopic examination was followed by gastric biopsies for specific culture of Helicobacter pylori (H.pylori) and antimicrobial susceptibility for isolated strains. Further molecular studies were performed for detection of clarithromycin resistance by PCR-RFLP for detection of 23S rRNA gene mutations. H.pylori was isolated from 68.8% of samples, figure 1. The isolates were with convergent rates from adults (62.2%)and children (68.4%). The isolated H.pylori had marked resistance for clarithromycin (46.2%) and amoxacillin (20.8%) with lower rates of resistance to tetracycline (15.4%) and metronidazole (12.8%). In comparison of antibiotics resistance among adults and children, there were insignificant difference between both groups regarding Clarithromycin (P=0.20) Amoxacillin (P=0.9), tetracyclin(P=0.08) and metronidazole (P=0.4). The remarkable finding is the significant increase in presence of mutations in isolates from adults compared to that from children (P=0.006). The most common type of mutation was for A2143G (53.4%) followed by A2142G (35.7%). Only 3 isolates could not be identified to have any of the two types of the studied mutations. We conclude from this study that clarithromycin resistance among H.pylori isolates is a common finding associated with specific point mutations of A2143G followed by A2142G. Resistance also to other antibiotics amoxicilline, tetracyline are common. Extended studies should be performed on large isolates from Egypt to elucidate the efficacy of first line triple therapy.


Keywords: Helicobacter pylori, Clarithromycin resistance, RFLP-PCR
Download this article as Download

How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 165-173. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.014