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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1074-1080
DOI: https://doi.org/10.20546/ijcmas.2019.808.125


Detection of E. coli from Sheep and Goat Faecal Samples and Identification by Conventional and Molecular Assay
K. Kumar, N. S. Sharma, Paviter Kaur* and A.K. Arora
Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, India
*Corresponding author
Abstract:

E. coli is a Gram negative bacteria belonging to enterobacteriacea family. They are commonly found in the intestine of animals and humans. Most E. coli are harmless but some can cause infection. In our study, we isolated E.coli from 100 (50 from sheep and 50 from goats) faecal samples of diarrhoeic as well as healthy sheep. Isolation was done on BHI, MLA and EMB agar. The isolated bacteria suspected for E. coli were subjected to biochemical tests viz catalase, oxidase and IMViC. The isolates confirmed by biochemical tests were further confirmed by genus specific PCR. Out of 100 samples, 40 samples were positive for presence of E. coli. On BHI creamy mucoid colonies were formed, while as on MLA lactose fermenting pink colonies were formed. On EMB, colonies with characteristic metallic sheen were formed. All the isolates were catalase positive, oxidase negative and gave a characteristic pattern in IMViC test i.e. indole positive, methyl red positive, Voges Prausker and citrate negative. The 40 isolates were subjected to genus specific PCR out of which 26 isolates were confirmed as E. coli.


Keywords: E. coli, Sheep, Goats, Biochemical test, PCR
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How to cite this article:

Kumar, K., N.S. Sharma, Paviter Kaur and Arora, A.K. 2019. Detection of E. coli from Sheep and Goat Faecal Samples and Identification by Conventional and Molecular Assay.Int.J.Curr.Microbiol.App.Sci. 8(8): 1074-1080. doi: https://doi.org/10.20546/ijcmas.2019.808.125