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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 787-797
DOI: https://doi.org/10.20546/ijcmas.2019.808.089


Acaricidal Activity of Crude Ethanolic Extract of Sphaeranthus indicus, its Fractions and Subfractions against Rhipicephalus (Boophilus) annulatus (Acari: Ixodidae)
Thirumangalath Meethal Divya1, Valliparampil Chandran Soorya2, Krishnan Kavillimakkil Amithamol2, Darsana Udayan1, Kanapadinchareveetil Sreelekha1, Suresh Narayanan Nair1, Karaparrambil Gopalan Ajithkumar2, Sanis Juliet1, Reghu Ravindran2* and Srikant Ghosh3
1Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi, P.O., Wayanad, Kerala 673 567, India
2Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi, P.O., Wayanad, Kerala 673 567, India
3Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India
*Corresponding author
Abstract:

Plants constitute a rich source of bioactive compounds and its acaricidal effect is increasingly been investigated as a potential solution against acaricide resistance. In the present study, the in vitro efficacy of crude ethanolic extract of leaves of Sphaeranthus indicus, its fractions (hexane, chloroform, butanol and water) and subfractions of the active acaricidal fraction were evaluated against Rhipicephalus (Boophilus) annulatus using the adult immersion technique (AIT). The percentage of adult mortality, inhibition of fecundity and hatching rate were assessed. The crude ethanolic extract of leaves of Sphaeranthus indicus revealed hundred per cent adult mortality and inhibition of fecundity at a concentration of 250 mg/mL. Among the four fractions of this extract tested, hexane fraction showed concentration dependent delayed adult tick mortality. At 10 per cent concentration of hexane fraction, 87.41 per cent mortality and 77.78 per cent inhibition of fecundity were observed. Among the 14 subfractions of the active hexane fraction, the subfraction 4 (at 2000 ppm) produced 45.83 per cent mortality and 41.06 per cent inhibition of fecundity. It may be concluded that the hexane fraction of the leaves of ethanolic extract of S. indicus and its subfraction 4 revealed significant acaricidal effects.


Keywords: Acaricidal effects. Rhipicephalus (Boophilus) annulatus. Sphaeranthus indicus. Ethanolic extract. Fractions, Adult immersion test
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How to cite this article:

Thirumangalath Meethal Divya, Valliparampil Chandran Soorya, Krishnan Kavillimakkil Amithamol, Darsana Udayan, Kanapadinchareveetil Sreelekha, Suresh Narayanan Nair, Karaparrambil Gopalan Ajithkumar, Sanis Juliet, Reghu Ravindran and Srikant Ghosh. 2019. Acaricidal Activity of Crude Ethanolic Extract of Sphaeranthus indicus, Its Fractions and Subfractions against Rhipicephalus (Boophilus) annulatus (Acari: Ixodidae).Int.J.Curr.Microbiol.App.Sci. 8(8): 787-797. doi: https://doi.org/10.20546/ijcmas.2019.808.089