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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 559-568
DOI: https://doi.org/10.20546/ijcmas.2019.808.067


Serosurveillance of Bovine Brucellosis Using RBPT and c-ELISA and Comparative Evaluation of Test Performance
Mugammad Umar Yuguda1, Sureshkannan Sundaram1*, Gunaseelan Lakshmanan2, Elango Ayyasamy3, Sridevi Purushothaman4, Porteen Kannan1 and Thiyagarajan Sanjeevi5
1Department of Veterinary Public Health and Epidemiology, Madras veterinary College, TANUVAS, Chennai, India
2Dean faculty of Basic Sciences (Retired) TANUVAS, Chennai, India
3PG Research Institute, TANUVAS, Kattuppakkam, Chennai, India
4Department of Clinics, Madras veterinary College, TANUVAS, Chennai, India
5Translational Research Platform for Veterinary Biologicals (TRPVB), TANUVAS, Chennai, India
*Corresponding author
Abstract:

The present study was conducted to evaluate the presence of Brucella infection amongst the randomly selected cattle and buffalo’s population in and around Chennai. The Rose Bengal Plate Test (RBPT) and Bru Alert® Brucella Antibody c-ELISA was employed to screen 355 sera samples (295 from cattle and 60 from buffalo) for the presence of Brucella antibody. The overall positivity of 8.73 % (31/355), 10.14 % (36/355) in bovine population, within this 9.15 % (27/295), 11.19 % (33/295) among white cattle and 6.66% (4/60), 5 % (3/60) among buffaloes by RBPT and c-ELSA respectively. Comparatively high seropositivity was found in >5 years age groups, females and exotic cross breed animals. The agreement between the two test was excellent (Kappa=0.8847) and also, chi-square test indicated an evidence of strong (P<0.01) association between the tests. Based on this study, c-ELISA has been found to be a gold standard test for detecting Brucella antibodies having more sensitivity and specificity. So on, it is recommended that RBPT could be successfully used for initial screening of brucellosis in bovines and c-ELISA should be used as confirmatory test to eliminate false positive results amongst positive sera.


Keywords: Bovine Brucellosis, RBPT, cELISA, Antibody detection tests, Abortion
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How to cite this article:

Mugammad Umar Yuguda, Sureshkannan Sundaram, Gunaseelan Lakshmanan, Elango Ayyasamy, Sridevi Purushothaman, Porteen Kannan and Thiyagarajan Sanjeevi. 2019. Serosurveillance of Bovine Brucellosis Using RBPT and c-ELISA and Comparative Evaluation of Test Performance.Int.J.Curr.Microbiol.App.Sci. 8(8): 559-568. doi: https://doi.org/10.20546/ijcmas.2019.808.067