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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 457-464
DOI: https://doi.org/10.20546/ijcmas.2019.808.052


Expression of GATA4 Gene into Differentiated Canine Induced Pluripotent Stem Cell
Purnima Singh, Tanmay Mondal, N. Mahalakshmi, Kuldeep Kumar, A.P. Madhusoodan, Mihir Sarkar and Sadhan Bag*
Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly (UP)-243122, India
*Corresponding author
Abstract:

Induced Pluripotent stem cells (iPSC) technology is involved in advancing the field of disease modelling and cell transplantation. Stem cell can differentiate under appropriate in vitro and in vivo conditions into different cell types. Under this study, we analysed the establishment of in vitro expression of GATA4 gene in cardiac differentiated canine iPSC. In vitro differentiation of canine iPSCs via embryoid bodies (EBs) was produced by ‘Hanging Drop’ method. EB’s were differentiated by using cardiac differentiation media. During differentiation, EBs was collected on day 4, 6, 8, 12, 16, 20 and 24 for characterization of cardiomyocytes specific marker expression. Total RNA from EBs was extracted by using the Trizol method and subsequently, cDNA was synthesized. The differentiated cells expressed cardiac-specific gene GATA 4 which started from day 4 of differentiation. Relative expression of cardiac-specific genes revealed that ciPSC has the potential to differentiate into cardiomyocytes which can be used for cardiac tissue regeneration and as disease models for pharmaceutical testing.


Keywords: Heart failure, Myocardium, Oxygen
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How to cite this article:

Purnima Singh, Tanmay Mondal, N. Mahalakshmi, Kuldeep Kumar, A.P. Madhusoodan, Mihir Sarkar and Sadhan Bag. 2019. Expression of GATA4 Gene into Differentiated Canine Induced Pluripotent Stem Cell.Int.J.Curr.Microbiol.App.Sci. 8(8): 457-464. doi: https://doi.org/10.20546/ijcmas.2019.808.052