National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Induced Pluripotent stem cells (iPSC) technology is involved in advancing the field of disease modelling and cell transplantation. Stem cell can differentiate under appropriate in vitro and in vivo conditions into different cell types. Under this study, we analysed the establishment of in vitro expression of GATA4 gene in cardiac differentiated canine iPSC. In vitro differentiation of canine iPSCs via embryoid bodies (EBs) was produced by ‘Hanging Drop’ method. EB’s were differentiated by using cardiac differentiation media. During differentiation, EBs was collected on day 4, 6, 8, 12, 16, 20 and 24 for characterization of cardiomyocytes specific marker expression. Total RNA from EBs was extracted by using the Trizol method and subsequently, cDNA was synthesized. The differentiated cells expressed cardiac-specific gene GATA 4 which started from day 4 of differentiation. Relative expression of cardiac-specific genes revealed that ciPSC has the potential to differentiate into cardiomyocytes which can be used for cardiac tissue regeneration and as disease models for pharmaceutical testing.
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