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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 175-180
DOI: https://doi.org/10.20546/ijcmas.2019.808.021


Comparison between Chromogenic Colour Agar and Biochemical Tests in the Identification of Causative Organism of Bacteriuria Associated with Urinary Schistosomiasis, in Abu Rukba Village, White Nile State (Sudan)
Husameldin Abdalla Bakhit1, Samah Sid Ahmed Mohamed Salih Elsafi2, Mohammed Baha Eldin Ahmed Saad3, Alaaeldeen Balal Ahmed4, Mohammed Eltoum Hamed Azoz5 and Mohammed A. Suliman6
1Faculty of Medical Laboratory Sciences, White Nile University, White Nile State, Kosti City, Sudan
2Department of Microbiology Laboratory, National Health Insurance, Microbiology Laboratory, White Nile State, Kosti City, Sudan
3Omdurman Ahlia Univesity, Faculty of Medical Laboratory Sciences, Omdurman, Sudan
4Department of Microbiology, White Nile University, Faculty of Medical Laboratory Sciences, White Nile State, Kosti City, Sudan
5Department of Surgery, Faculty of Medicine, University of El Imam El Mahdi, White Nile State, Kosti City, Sudan
6Department of Parasitology and Medical Entomology, Faculty of Medical Laboratory Sciences, University of El Imam El Mahdi, White Nile State, Kosti City, Sudan
*Corresponding author
Abstract:

Chromogenic medium is culture media for simple and fast detection of bacteria using chromogenic substrates, resulting in the different coloration of certain bacteria colonies. Have several advantages over traditional media, such as cysteine-lactose-electrolyte deficient (CLED) medium, allow for easier recognition of mixed growth, save time, reduce workload and provides higher detection rates. To compare between chromogenic colour agar and biochemical tests in identification of organisms causing bacteriuria that associated with urinary Schistosomiasis. The study was conducted in Abu Rukba village in the White Nile State, Sudan. The study was carried out on the entire population of the village (1029 individuals) during the period of November 2011 and November 2013. Urine samples were collected from study population after having their informed consent, school staff and local administrators. A total of 1029 Mid-stream urine specimens were collected aseptically as possible, in a sterile wide mouth container in the morning and processed by the laboratory within 2 hours of collection, or were kept refrigerated at 4ºC until delivery to the laboratory and were processed no longer than 4 hours after collection. Each specimen was routinely processed using the centrifugation technique to detect Schistosoma and cultivated by using chromogenic agar and Cystine Lactose Electrolyte Deficient agar plate (CLED) then identified by biochemical tests. Antibiotic susceptibility also was done. Bacterial culture for those who were positive for S. haematobium infection (594) revealed growth of Staphylococcus aureus in 116 urine samples (19.5%), Enterococcus faecalis in 57 (9.6%), Escherichia coli in 28 (4.7%), Klebsiella pneumoniae in 20 (3.4%), Proteus species in 2 (0.4%) and mixed infection in 71 (11.9%). Comparing the chromogenic media results with the biochemical reaction results, 95.5% of all isolates were recovered on both. The remaining 4.5% of isolates were different by both tests. Chromogenic medium provided a reliable alternative to CLED medium and biochemical tests for the routine culture of urine specimens.


Keywords: Urinary Schistosomiasis, Bacteriuria, Chromogenic medium, Biochemical tests
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How to cite this article:

Husameldin Abdalla Bakhit, Samah Sid Ahmed Mohamed Salih Elsafi, Mohammed Baha Eldin Ahmed Saad, Alaaeldeen Balal Ahmed, Mohammed Eltoum Hamed Azoz and Mohammed A. Suliman. 2019. Comparison between Chromogenic Colour Agar and Biochemical Tests in the Identification of Causative Organism of Bacteriuria Associated with Urinary Schistosomiasis, in Abu Rukba Village, White Nile State (Sudan).Int.J.Curr.Microbiol.App.Sci. 8(8): 175-180. doi: https://doi.org/10.20546/ijcmas.2019.808.021