Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3459-3466
DOI: https://doi.org/10.20546/ijcmas.2019.802.405


Effect of Season on the Prevalence of Virulence Genes of V. parahaemolyticus in Molluscan Shellfish along the Thoothukudi Coast, India
V. Alamelu*, G. Jeyasekaran, R. Shalini and R. Jeya Shakila
Department of Fish Quality Assurance and management, Fisheries College and Research Institute, Thoothukudi, India
*Corresponding author
Abstract:

Commercially available molluscan shellfish such as oysters and clams were collected monthly along the Rochepark, Thoothukudi and brought to the laboratory in moist condition immediately after harvesting and analysed for the prevalence of virulence genes of V. parahaemolyticus. The meat was carefully shucked (meat and fluid) from a pool of 6 – 8 oysters/clams and used. Prevalence of tdh, trh genes of V. parahaemolyticus was confirmed by PCR as well as Direct plating method (TCBS). The present study revealed that the effect of season on the presence of virulence genes of V. parahaemolyticus was insignificant compared to other reports.The result of this study shows less prevalence of pathogenic V. parahaemolyticus and moderate to high prevalence of non-pathogenic V. parahaemolyticus in both oysters and clams during summer season followed by winter season. It could also be observed that 2% Crassostrea sp. showed positive for pathogenic (tdh) V. parahaemolyticus and 61% Crassostrea sp. and 51.2% of Meretrix sp. from HB showed positive for non-pathogenic V. parahaemolyticus.


Keywords: V. parahaemolyticus, Molluscan shellfish, Virulence genes, PCR
Download this article as Download

How to cite this article:

Alamelu V., G. Jeyasekaran, R. Shalini and Jeya Shakila R. 2019. Effect of Season on the Prevalence of Virulence Genes of V. parahaemolyticus in Molluscan Shellfish along the Thoothukudi Coast, India.Int.J.Curr.Microbiol.App.Sci. 8(2): 3459-3466. doi: https://doi.org/10.20546/ijcmas.2019.802.405