Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 2249-2254
DOI: https://doi.org/10.20546/ijcmas.2019.807.274


Cross-specific Steroidogenic Enzyme Genes Expression during Gonadal Development in Lepidocephalus thermalis
Balaganesan Mariappan1*, Felix Nathan2 and Krithika Rajendran3
1Fisheries College and Research Institute, Thoothukudi, TamilNadu Dr. J. Jayalalithaa Fisheries University-Nagapattinam 611002, India
2Directorate of Incubation and Vocational Training in Aquaculture, ECR – Muttukadu, Kancheepuram 603112, Kanchipuram
3Anna University, ACT Campus, Chennai 600025, India
*Corresponding author
Abstract:

Sex steroids were involved during the process of sex differentiation, gametogenesis and sex reversal in fish and the expression of steroidogenic enzyme genes and their related transcription factors are critical for the development of the gonads and the regulation of steroidogenesis. The early stage of steroidogenesis is important for biosynthesis of testosterone and estradiol. In this study, three steroidogenic enzyme genes cyp19a1, 11βhsd (11β-hydroxysteroid dehydrogenase) and 3βhsd (3β-hydroxysteroid dehydrogenase) expression in various stages of gonad development has been analysed. As this is the first recordical study of steroidogenic enzyme genes in L. thermalis, the primers of Air breathing catfish, Clarias gariepinus for the genes cyp19a1, 11βhsd and 3βhsd has been used to find the expression level at various stages. Based on the previous studies in catfish in these genes, cyp19a1 is an ovarian aromatase found abundant in differentiating female gonads. 11βhsd has higher expression in the spawning phase of reproductive cycle and transactivates to regulate male reproduction in teleost. 3βhsd is important in both spermatogenesis and oogenesis. From similar experimental methods performed in C. gariepinus the expression level of these genes in L. thermalis is observed. From the graph, it is evident that in L. thermalis, higher expression level by cyp19a1, 11βhsd and 3βhsd is in medium sized testis than in the other. Hence these genes play a vital role in spermatogenesis in L. thermalis.


Keywords: Steroidogenic Enzyme, cyp19a1, 11βhsd, 3βhsd, L. thermalis
Download this article as Download

How to cite this article:

Balaganesan Mariappan, Felix Nathan and Krithika Rajendran. 2019. Cross-specific Steroidogenic Enzyme Genes Expression during Gonadal Development in Lepidocephalus thermalis.Int.J.Curr.Microbiol.App.Sci. 8(7): 2249-2254. doi: https://doi.org/10.20546/ijcmas.2019.807.274