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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 2135-2143
DOI: https://doi.org/10.20546/ijcmas.2019.807.257


Aerobic Bacteriological Profile of Chronic Suppurative Otitis Media and Special Reference to Methicillin Resistant Staphylococcus aureus
Jyoti R. Hundekar*, Surekha, Y.A. Mariraj Jeer and S. Krishna
Department of Microbiology, Vijayanagara Institute of Medical Sciences, Bellary, India
*Corresponding author
Abstract:

Chronic Suppurative Otitis Media (CSOM) is a well known disease for its persistence and recurrence in spite of treatment. It was found to be the single major cause of conductive deafness, speech disorders. It involves considerable morbidity and can cause extra and intracranial complications. Due to development of beta lactamase producing microorganisms, drug resistant strains emerge making drug resistance a major cause of treatment failure. The isolation of Methicillin Resistant Staphylococcus aureus (MRSA) was reported within 1year of introduction of methicillin. In many hospitals 40-50% of Staphylococcus aureus isolates are now resistant to methicillin. Out of 129 ear discharge samples, 86 samples showed growth, the isolates were identified by conventional microbiological methods including colony morphology and standard biochemical reactions. Antibiotic susceptibility tests were performed by Kirby-Bauer disk diffusion method. MRSA in isolated S. aureus was detected by cefoxitin disk diffusion method. The MIC(Minimum inhibitory concentration) was determined by agar dilution method and Etest. Among 129 ear samples, 100 bacterial isolates were isolated, the most common organism isolated was Staphylococcus aureus 36 (36%) followed by Pseudomonas spp. 34 (34%), Coagulase negative Staphylococcus 9(9%). Others include, E. coli, Acinetobacter spp, Citrobacter spp, Streptococcus spp (4%) each. Klebsiella spp and Proteus vulgaris 2(2%) each was isolated and Enterobacter spp, in one sample (1%) isolated. The antibiotic susceptibility testing showed Amikacin as the most effective drug followed by, gentamicin, cefotaxime, cotrimoxazol, ciprofloxacin and amoxyclav.


Keywords: Bacteriological profile, Chronic suppurative otitis media.
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How to cite this article:

Jyoti R. Hundekar, Surekha, Y.A. Mariraj Jeer and Krishna, S. 2019. Aerobic Bacteriological Profile of Chronic Suppurative Otitis Media and Special Reference to Methicillin Resistant Staphylococcus aureus.Int.J.Curr.Microbiol.App.Sci. 8(7): 2135-2143. doi: https://doi.org/10.20546/ijcmas.2019.807.257